| Full text | |
| Author(s): |
Campos, Rafael
;
Niederauer, Ana Julia Schmidt
;
Britto-Junior, Jose
;
de Souza, Valeria B.
;
Schenka, Andre A.
;
Monica, Fabiola Z.
;
Moraes, Manoel Odorico
;
Moraes, Maria Elisabete A.
;
Antunes, Edson
;
De Nucci, Gilberto
Total Authors: 10
|
| Document type: | Journal article |
| Source: | COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY; v. 285, p. 13-pg., 2024-08-17. |
| Abstract | |
Mammalian and reptilian vascular tissues present basal release of 6-nitrodopamine, which is reduced when the tissues are pre-incubated with the NO synthase inhibitor L-NG-Nitro arginine methyl ester (L-NAME), or when the endothelium is mechanically removed. 6-Nitrodopamine induces vasorelaxation in pre-contracted vascular rings by antagonizing the dopaminergic D2-like receptor. Here it was investigated whether male swine vessels (including carotid, left descendent coronary, renal, and femoral arteries) release 6-nitrodopamine, dopamine, noradrenaline, and adrenaline, as measured by liquid chromatography coupled to tandem mass spectrometry. The in vitro vasorelaxant action of 6-nitrodopamine was evaluated in carotid, coronary, renal, and femoral arteries precontracted by U-46619 (3 nM), and compared to that induced by the dopamine D2-receptor antagonist L-741,626. Expression of tyrosine hydroxylase and the neuromaker calretinin was investigated by immunohistochemistry. All vascular tissues presented basal release of endothelium-derived catecholamines. The relaxation induced by 6-nitrodopamine was not affected by preincubation of the tissues with either L-NAME (100 mu M, 30min preincubation) or the heme-site inhibitor of soluble guanylyl cyclase ODQ (100 mu M, 30-min preincubation). Electrical field stimulation (EFS)-induced contractions were significantly potentiated by previous incubation with L-NAME, but unaffected by ODQ preincubation. The contractions induced by EFS were reduced by preincubation with either 6-nitrodopamine or L-741,626. Immunohistochemistry in all arteries revealed the presence of tyrosine hydroxylase in the endothelium, whereas immunoreactivity for calretinin was negative. Swine vessels present basal release of endothelium-derived catecholamines and expression of tyrosine hydroxylase in the endothelium. The vasodilation induced by 6-nitrodopamine is due to blockade of dopaminergic D2-like receptors. (AU) | |
| FAPESP's process: | 21/13726-6 - Identification of molecules related to the synthesis of adrenaline, noradrenaline and dopamine in the endothelium of reptiles and mammals by immunohistochemistry and in situ hibridization |
| Grantee: | Valéria Barbosa de Souza |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 22/08232-7 - Pharmacological characterization of 6-Nitrodopamine in the systemic and renal hemodynamics of mammals and reptiles |
| Grantee: | Rafael de Morais Campos |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 17/15175-1 - Modulation of soluble guanylate cyclase and the intracellular levels of cyclic nucleotides in the lower urinary tract and prostate |
| Grantee: | Edson Antunes |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 19/16805-4 - Evaluation of the pathophysiological role of endothelial catecholamines |
| Grantee: | Gilberto de Nucci |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 21/14414-8 - Evaluation of the action of nitro-catecholamines in the cardiovascular system |
| Grantee: | José Britto Júnior |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 23/15350-9 - 6-Nitrodopamine and the respiratory system |
| Grantee: | Rafael de Morais Campos |
| Support Opportunities: | Scholarships abroad - Research Internship - Post-doctor |
| FAPESP's process: | 23/14557-9 - The transduction mechanism(s) employed by 6-nitrodopamine and 6-cyanodopamine in human platelets and cultured cell lines |
| Grantee: | José Britto Júnior |
| Support Opportunities: | Scholarships abroad - Research Internship - Post-doctor |