| Full text | |
| Author(s): |
Valente, Roniele Santana
;
Marsico, Thamiris Vieira
;
Maiollo, Bruna Aparecida Prado
;
Lopes, Nelyane Jose
;
Tannura, Juliana Hayashi
;
Sudano, Mateus Jose
Total Authors: 6
|
| Document type: | Journal article |
| Source: | Theriogenology; v. 215, p. 6-pg., 2023-12-08. |
| Abstract | |
In this study, we investigated the impact of incorporating Interleukin-13 (IL-13) into the embryonic culture medium and its influence on cryotolerance and cellular viability of vitrified bovine embryos. Two distinct time points for IL-13 supplementation were explored: during the final hours of culture prior to cryopreservation and during the period of recultivation following cryopreservation and warming. Cryosurvival rates, total cell count, and cell viability were assessed using the TUNEL technique to determine the apoptotic percentage. Re-expansion and hatching rates did not show differences among all groups (P > 0.05), and the total cell number was com-parable between the treated and control groups (P > 0.05). However, the group that received IL-13 before vitrification exhibited a higher apoptotic percentage (P < 0.05). This suggests that the anti-inflammatory effect of IL-13 may have impacted the embryo's defense capacity against the stress induced by cryopreservation, leading to an increased percentage of apoptosis, although it did not influence the developmental resumption capability. (AU) | |
| FAPESP's process: | 21/01446-9 - Multi-user Equipment approved in grant 18/21496-8: Inverted Microscope Equipped with Fluorescence and Differential interference Contrast (DIC) |
| Grantee: | Mateus José Sudano |
| Support Opportunities: | Multi-user Equipment Program |
| FAPESP's process: | 19/17796-9 - Simultaneous biopsy and lipid profile of embryo inner cell mass and trofectoderm |
| Grantee: | Thamiris Vieira Marsico |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 18/21496-8 - Pathways for embryo-derived pregnancy |
| Grantee: | Mateus José Sudano |
| Support Opportunities: | Research Grants - Young Investigators Grants |
| FAPESP's process: | 19/18415-9 - Role of inflammatory pathways on embryonic competence and cryotolerance |
| Grantee: | Roniele Santana Valente |
| Support Opportunities: | Scholarships in Brazil - Doctorate |