| Full text | |
| Author(s): |
Kowaltowski, Alicia J.
;
Abdulkader, Fernando
Total Authors: 2
|
| Document type: | Journal article |
| Source: | BIOPHYSICAL JOURNAL; v. 123, n. 24, p. 8-pg., 2024-12-17. |
| Abstract | |
The scientific literature on mitochondria has increased significantly over the years due to findings that these organelles have widespread roles in the onset and progression of pathological conditions such as metabolic disorders, neurodegenerative and cardiovascular diseases, inflammation, and cancer. Researchers have extensively explored how mitochondrial properties and functions are modified in different models, often using fluorescent inner mitochondrial membrane potential (AWm) probes to assess functional mitochondrial aspects such as protonmotive force and oxidative phosphorylation. This review provides an overview of existing techniques to measure ApH and AWm, highlighting their advantages, limitations, and applications. It discusses drawbacks of AWm probes, especially when used without calibration, and conditions where alternative methods should replace AWm measurements for the benefit of the specific scientific objectives entailed. Studies investigating mitochondria and their vast biological roles would be significantly advanced by the understanding of the correct applications as well as limitations of protonmotive force measurements and use of fluorescent AWm probes, adopting more precise, artifactfree, sensitive, and quantitative measurements of mitochondrial functionality. (AU) | |
| FAPESP's process: | 20/06970-5 - Mitochondrial ion transporters as sensors and regulators in energy metabolism |
| Grantee: | Alicia Juliana Kowaltowski |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 13/07937-8 - Redoxome - Redox Processes in Biomedicine |
| Grantee: | Ohara Augusto |
| Support Opportunities: | Research Grants - Research, Innovation and Dissemination Centers - RIDC |