| Full text | |
| Author(s): |
Monesi, Nadia
;
Fernandes, Guilherme Magre
;
Valer, Felipe Berti
;
Uliana, Joao Vitor Cardoso
;
Trinca, Vitor
;
Azzolini, Ana Elisa Caleiro Seixas
;
Gorab, Eduardo
;
Alberici, Luciane Carla
Total Authors: 8
|
| Document type: | Journal article |
| Source: | Biochimie; v. 233, p. 15-pg., 2025-06-01. |
| Abstract | |
Alternative oxidase (AOX) (EC 1.10.3.11) is a terminal oxidase in the mitochondrial inner membrane that branches the canonical electron transport system (ETS). AOX is ubiquitous in plants, frequently found in fungi and protists and presents a more sporadic distribution in metazoans. More recently, AOX has gained attention due to its potential application in gene therapy for treatment of mitochondrial diseases. Here we characterized the AOX in the basal Dipteran, Pseudolycoriella hygida using a combination of genomic analyses, molecular, functional and in vivo survival assays. AOX is a single copy gene that encodes three developmental stage specific protein isoforms. AOX localizes to the mitochondria in adult thoracic muscles, which present cyanide-resistant respiration that is sensitive to the AOX inhibitor salicylhydroxamic acid (SHAM). Both the cyanide-resistant respiration and AOX levels gradually increase during aging, but are not influenced by thermal stress. Thoracic mitochondria respire using substrates derived from several metabolic routes, such as pyruvate, proline, acylcarnitine, NADH and glycerol-3P, and present values of oxidative phosphorylation capacity ((P-L)/E 1/4 0.70) and coupling (P/L 1/4 4.35; L/ E 1/4 0.21). Adult flies exhibit a high survival resistance for SHAM-sensitive complex III inhibition. Together, our results demonstrate the presence of a functional AOX in a terrestrial arthropod and provide insights regarding AOX function in animals and evolution of respiratory systems in metazoans. Psl. hygida emerges as a natural and valuable model for comprehensive AOX research at the whole-organism level which complements models expressing the heterologous enzyme. (c) 2025 Elsevier B.V. and Soci & eacute;t & eacute; Fran & ccedil;aise de Biochimie et Biologie Mol & eacute;culaire (SFBBM). All rights are reserved, including those for text and data mining, AI training, and similar technologies. (AU) | |
| FAPESP's process: | 17/02588-6 - Molecular aspects of chromosome end regions in Sciaridae dipterans |
| Grantee: | Eduardo Gorab |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 16/25325-8 - The dipteran protoracic glands: morphological, genomic and functional studies in the dipteran B. hygida and D. melanogaster |
| Grantee: | Nadia Monesi |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 18/10089-2 - Neural, hormonal and nutritional control of autophagy |
| Grantee: | Isis Do Carmo Kettelhut |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 21/11527-6 - Drosophila melanogaster as a molecular modulation model for in vivo study of autophagy and its relationship with mitochondrial metabolism |
| Grantee: | Felipe Berti Valer |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |