Assessment of products generated by photodynamic and sonodynamic action via Liquid Chromatography - Tandem Mass Spectrometry

Author(s)

Ponce Ayala, Erika Toneth ; Barreiro, Juliana Cristina ; Silva e Carvalho, Iago ; Antunes, Camila Aparecida ; Alves, Fernanda ; Pratavieira, Sebastiao

• Document type: Journal article
• Source: 2024 SBFOTON INTERNATIONAL OPTICS AND PHOTONICS CONFERENCE, SBFOTON IOPC 2024; v. N/A, p. 3-pg., 2024-01-01.

Abstract

Photodynamic and sonodynamic action rely on the interaction of a sensitizing molecule with light and ultrasound, respectively, generating reactive oxygen species. These interactions also lead to the generation of other products, which might be cytotoxic or photoactive. Identifying them may help to understand the mechanisms involved in each action. So, detecting and characterizing the products become very important. This work aimed to identify the products generated by photodynamic and sonodynamic action and discuss the degradation mechanisms associated with sonodynamic action. The well-known organic sensitizers, Protoporphyrin IX, Methylene Blue, Curcumin, and Chlorin e6 were used to mediate photodynamic and sonodynamic action. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used as the detection method. The detected ions' mass/charge ratio and retention time were summarized. (AU)

• FAPESP's process: 21/14446-7 - Multi-user equipment approved in grant 13/07276-1: Mass spectrometer with triple quadrupole analyzer and gas chromatography system coupled to the mass spectrometer
• Grantee: Vanderlei Salvador Bagnato
• Support Opportunities: Multi-user Equipment Program
• FAPESP's process: 13/07276-1 - CEPOF - Optics and Photonic Research Center
• Grantee: Vanderlei Salvador Bagnato
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
• FAPESP's process: 14/50857-8 - National Institute in Basic Optics and Applied to Life Sciences
• Grantee: Vanderlei Salvador Bagnato
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 23/14868-4 - Understanding the basic mechanisms of ultrasound interaction with sensitizers and biological tissues to improve the sonodynamic therapy.
• Grantee: Sebastião Pratavieira
• Support Opportunities: Regular Research Grants