Insights into the use of DNA content in head and neck squamous cell carcinoma as a method for patient stratification and targeted therapy: Revisiting old concepts and exploring new possibilities

Author(s)

Goncalves, Moises Willian Aparecido ; Maciel, Figueiredo ; Lavareze, Luccas ; Egal, Erika Said Abu ; Altemani, Albina ; Sperandio, Marcelo ; Mariano, Fernanda Viviane

• Document type: Journal article
• Source: JOURNAL OF STOMATOLOGY ORAL AND MAXILLOFACIAL SURGERY; v. 126, n. 3, p. 7-pg., 2025-06-01.

Abstract

Background: This review aimed to emphasize the implications of DNA content in head and neck squamous cell carcinoma (HNSCC), focusing on its predictive value, role in patient stratification, and potential as a therapeutic target for this malignancy. Methods: A narrative review of the literature was conducted through electronic database searches. Results: In conventional HNSCC, aneuploid tumors are associated with increased lymph node metastasis, locoregional recurrences, poor response to radiotherapy and chemotherapy, and worse prognosis. Few studies specifically address the role of DNA content in young HNSCC patients. These studies reveal that young patients exhibit high DNA content abnormalities, suggesting significant genomic instability and potential genetic differences compared to older patients. Regarding HPV and DNA content, no difference was found between HPV-associated and HPV-independent tumors. More research is needed to understand the role of DNA content in histological subtypes, surgical margins, and targeted therapy. Conclusion: This review highlights the findings related to DNA content in HNSCC, suggesting its usefulness in patient stratification and outcome prediction.<br /> (c) 2025 Elsevier Masson SAS. All rights are reserved, including those for text and data mining, AI training, and similar technologies. (AU)

• FAPESP's process: 23/14770-4 - DNA content analysis of different histological subtypes and epidemiological groups of head and neck squamous cell carcinomas and their association to the tumor microenvironment
• Grantee: Moisés Willian Aparecido Gonçalves
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 22/11861-6 - Comparative analysis of Adenoid Cystic Carcinoma exome in xenograft model with and without entinostat and cisplatin therapy
• Grantee: Luccas Lavareze dos Reis
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 17/06579-1 - Predictive value of DNA ploidy combined with malignant transformation biomarkers in oral potentially malignant diseases
• Grantee: Marcelo Sperandio
• Support Opportunities: Regular Research Grants