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Unveiling the toxicity mechanism of copper tungstate (CuWO4) particles on the microalga Raphidocelis subcapitata assessed by multiple endpoints

Full text
Author(s):
Gebara, Renan Castelhano ; de Abreu, Cinthia Bruno ; Rocha, Giseli Swerts ; Assis, Marcelo ; de Oliveira, Marisa Carvalho ; Ribeiro, Renan Augusto Pontes ; Melao, Maria da Graca Gama ; Longo, Elson
Total Authors: 8
Document type: Journal article
Source: Environmental Pollution; v. 386, p. 9-pg., 2025-12-01.
Abstract

Semiconductors have multiple applications in society, including photocatalysis and electrochemical sensors. Copper tungstate (CuWO4) particles can degrade organic compounds from water, but their deleterious effects on aquatic biota must be evaluated before their widespread use. We aimed to assess the toxicity of CuWO4 on the standardized microalga Raphidocelis subcapitata using a multiparameter approach. Our study comprehends the analysis of growth, lipid classes, carbohydrates, chlorophyll a, ROS production, photosynthesis, cell morphology, and theoretical calculations, to elucidate the toxicity mechanisms of this compound. We found that CuWO4 affected the growth at 2.8 mg/L, followed by an increase in cell granularity and volume at 2.8 and 5.6 mg/L, respectively. We also observed increased intracellular ROS production, photosynthesis impairment, and changes in lipid classes (especially triacylglycerol - TAG, with potential to biofuels), carbohydrates and chlorophyll a content. Besides the toxic effects caused by copper ion release and the physical and chemical effects of the particles, experimental and theoretical chemical analysis showed that the semiconductor CuWO4 itself produces oxidative species, especially photogenerated h+ (holes), and hydroxyl (center dot OH) and superoxide (center dot O2-) radicals, which can be associated with the localization of electronic levels summed to the undercoordination degree of the exposed surfaces. Our study shows that although CuWO4 is promisor in water decontamination, it is toxic to a green microalga at the base of aquatic food chains, being a possible threat to these ecosystems. Further ecotoxicological studies with other aquatic organisms is highly advised to better understand the effects of this semiconductor on entire environments. (AU)

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Grantee:Marcelo de Assis
Support Opportunities: Scholarships in Brazil - Post-Doctoral
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Grantee:Hugo Miguel Preto de Morais Sarmento
Support Opportunities: Research Grants - Young Investigators Grants
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Grantee:Renan Castelhano Gebara
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 13/07296-2 - CDMF - Center for the Development of Functional Materials
Grantee:Elson Longo da Silva
Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
FAPESP's process: 25/08354-3 - Evaluating the toxicity of Cobalt Tungstate Nanoparticles (CoWO4-NPs) and Nickel Tungstate Nanoparticles (NiWO4-NPs) to Danio rerio embryos
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Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
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Support Opportunities: Scholarships abroad - Research Internship - Post-doctor