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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Differential regulation of MMP-13 expression in two models of experimentally induced periodontal disease in rats

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Author(s):
de Aquino, Sabrina Garcia [1] ; Guimaraes, Morgana Rodrigues [1] ; Stach-Machado, Dagmar Ruth [2] ; Francisco da Silva, Juliete Aparecida [2] ; Spolidorio, Luis Carlos [3] ; Rossa, Jr., Carlos [1]
Total Authors: 6
Affiliation:
[1] State Univ Sao Paulo UNESP, Sch Dent Araraquara, Dept Diag & Surg, Araraquara, SP - Brazil
[2] State Univ Campinas UNICAMP, Inst Biol, Dept Microbiol & Immunol, Campinas, SP - Brazil
[3] State Univ Sao Paulo UNESP, Sch Dent Araraquara, Dept Physiol & Pathol, Araraquara, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: ARCHIVES OF ORAL BIOLOGY; v. 54, n. 7, p. 609-617, JUL 2009.
Web of Science Citations: 15
Abstract

Objective: Evaluate expression of MMP-13 during the course of two models experimentally induced periodontal disease in rats. Design: Expression of MMP-13 at mRNA and protein levels was studied, respectively, by reverse transcription polymerase chain reaction (RT-PCR) and Western blotting. Two experimental models were used: LPS injections and ligature placement. 30 jig of LPS from Eschericia coli was injected twice a week into the palatal aspect of upper molars. Ligatures were placed at the gingival margin around lower first molars. Controls received injections of PBS vehicle and no ligatures on lower molars. Samples were collected 5,15 and 30 days after initiation of periodontal disease and processed for extraction of total RNA, total protein, and routinely processed for histology. Results: Both experimental models produced a significant increase on the inflammatory infiltrate that paralleled elevated levels of MMP-13 mRNA and protein at 5 and 15 days. The LPS model was associated with a sustained level of inflammation and increased MMP-13 mRNA throughout the 30 days, whereas the ligature model showed a decrease on the severity of inflammation and MMP-13 mRNA at the 30-day period. interestingly, MMP-13 protein levels were diametrically contrary to the mRNA levels. Conclusion: MMP-13 expression during LPS- and ligature-induced experimental periodontal disease follows the increase on severity of inflammation at the earliest periods. At 30 days, there is a decrease on the severity of inflammation on the ligature model associated with decreased MMP-13 mRNA. There is a lack of transcription-translation coupling of MMP-13 gene in both experimental models. (C) 2009 Elsevier Ltd. All rights reserved. (AU)

FAPESP's process: 06/07283-4 - Effect of IL-2 inhibition by FK-506 on RANKL and OPG gene expression in ligature-induced periodontal disease: an in vitro and in vivo study
Grantee:Angélica Martins
Support Opportunities: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 05/04428-9 - Effect of IL-2 inhibition by FK-506 on RANKL and OPG gene expression in ligature-induced periodontal disease: an in vitro and in vivo study
Grantee:Carlos Rossa Junior
Support Opportunities: Regular Research Grants