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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Crystallization and Preliminary Crystallographic Analysis of Laminarinase from Rhodothermus marinus: A Case of Pseudomerohedral Twinning

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Author(s):
Golubev, Alexander M. [1, 2] ; Rojas, Adriana L. [1] ; Nascimento, Alessandro S. [1] ; Bleicher, Lucas [1] ; Kulminskaya, Anna A. [2] ; Eneyskaya, Elena V. [2] ; Polikarpov, Igor [1]
Total Authors: 7
Affiliation:
[1] Univ Sao Paulo, Inst Fis Sao Carlos, BR-13560970 Sao Carlos, SP - Brazil
[2] Petersburg Nucl Phys Inst, St Petersburg 188300 - Russia
Total Affiliations: 2
Document type: Journal article
Source: PROTEIN AND PEPTIDE LETTERS; v. 15, n. 10, p. 1142-1144, OCT 2008.
Web of Science Citations: 1
Abstract

Thermophilic endo-1,3(4)-beta-glucanase (laminarinase) from Rhodothermus marinus was crystallized by the hanging-drop vapor diffusion method. The needle-like crystals belong to space group P2(1) and contain two protein molecules in the asymmetric unit with a solvent content of 51.75%. Diffraction data were collected to a resolution of 1.95 angstrom and resulted in a dataset with an overall R-merge of 10.4% and a completeness of 97.8%. Analysis of the structure factors revealed pseudomerohedral twinning of the crystals with a twin fraction of approximately 42%. (AU)

FAPESP's process: 06/00182-8 - Structural biophysics of nuclear receptors and related proteins
Grantee:Igor Polikarpov
Support type: Research Projects - Thematic Grants