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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Prolactin-modulated gene expression profiles in pancreatic islets from adult female rats

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Author(s):
Bordin, Silvana [1] ; Amaral, Maria E. C. ; Anhê, Gabriel F. ; Delghingaro-Augusto, Viviane ; Cunha, Daniel A. ; Nicoletti-Carvalho, José E. ; Boschero, Antonio Carlos
Total Authors: 7
Affiliation:
[1] Universidade de São Paulo (USP). Instituto de Ciências Biomédicas - Brasil
Total Affiliations: 7
Document type: Journal article
Source: Molecular and Cellular Endocrinology; v. 220, n. 1/2, p. 41-50, May 2004.
Field of knowledge: Biological Sciences - Physiology
Abstract

The effects of prolactin (PRL) on transcript profile expression in 24 h cultured pancreatic adult rat islets were investigated by cDNA expression array analysis to identify possible candidate mRNA species that encode proteins involved in the maturation and growth of the endocrine pancreas. The expression of 54 out of 588 genes was altered by treatment with PRL. The differentially expressed transcripts identified were distributed in six main categories involved in cell proliferation and differentiation, namely, cell cycle regulation, signal transduction, transcription factors and coactivators, translational machinery, Ca2+-mediated exocytosis, and immuno-response. Treatment with PRL also reduced the expression of genes related to apoptosis. Several genes, whose expression was previously not known to be modulated by PRL were also identified including macrophage migration inhibitory factor and Ca2+/calmodulin-dependent protein kinase IV. These genes have recently been shown to play a crucial role in insulin secretion and insulin gene expression, respectively. Treatment with PRL also modified the expression of AKT2 and bone morphogenetic protein receptor 1A that control glucose homeostasis and directly affect the behavior of endocrine pancreas and/or the sensitivity of target tissues to insulin. In conclusion, PRL induces several patterns of gene expression in pancreatic islet cells. The analysis of these different patterns will be useful for understanding the complex mechanism of action of PRL in the maturation and differentiation of pancreatic islets.(AU)