Advanced search
Start date
Betweenand
(Reference retrieved automatically from Google Scholar through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Differential gene expression profiling in mucus glands of honey bee (Apis mellifera) drones during sexual maturation

Full text
Author(s):
Colonello-Frattini‚ N.A. ; Hartfelder‚ K.
Total Authors: 2
Document type: Journal article
Source: Apidologie; v. 40, n. 4, p. 481-495, 2009.
Abstract

The mating sign that each drone leaves when mating with a queen essentially consists of mucus gland proteins. We employed a Representational Difference Analysis (RDA) methodology to identify genes that are differentially expressed in mucus glands during sexual maturation of drones. The RDA library for mucus glands of newly emerged drones was more complex than that of 8 day-old drones, with matches to 20 predicted genes. Another 26 reads matched to the Apis genome but not to any predicted gene. Since these ESTs were located within ORFs they may represent novel honey bee genes, possibly fast evolving mucus gland proteins. In the RDA library for mucus glands of 8 day-old drones, most reads corresponded to a capsid protein of deformed wing virus, indicating high viral loads in these glands. The expression of two genes encoding venom allergens, acid phosphatase-1 and hyaluronidase, in drone mucus glands argues for their homology with the female venom glands, both associated with the reproductive system. (AU)

FAPESP's process: 99/00719-6 - Africanized honeybees: an integrated analysis of the africanization process of Apis mellifera, focusing on determinants in fertility of drones, queens and workers
Grantee:Zilá Luz Paulino Simões
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 05/03926-5 - Functional genomics of Apis mellifera: search for new genes and functional networks in the context of development, different castes and reproduction
Grantee:Zilá Luz Paulino Simões
Support Opportunities: Research Projects - Thematic Grants