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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Chromosomal studies in four species of genus Chaunus (Bufonidae, Anura): localization of telomeric and ribosomal sequences after fluorescence in situ hybridization (FISH)

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Author(s):
Amaro-Ghilardi, Renata Cecilia [1, 2] ; de Jesus Silva, Maria Jose [3] ; Rodrigues, Miguel Trefaut [1] ; Yonenaga-Yassuda, Yatiyo [2]
Total Authors: 4
Affiliation:
[1] Univ Sao Paulo, Inst Biociencias, Dept Zool, BR-05508900 Sao Paulo - Brazil
[2] Univ Sao Paulo, Inst Biociencias, Dept Genet & Biol Evolutiva, BR-05508900 Sao Paulo - Brazil
[3] Inst Butantan, Genet Lab, BR-05508900 Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Genetica; v. 134, n. 2, p. 159-168, Oct. 2008.
Field of knowledge: Biological Sciences - Genetics
Web of Science Citations: 11
Abstract

Fluorescence in situ hybridization (FISH) using telomeric and ribosomal sequences was performed in four species of toad genus Chaunus: C. ictericus, C. jimi, C. rubescens and C. schneideri. Analyses based on conventional, C-banding and Ag-NOR staining were also carried out. The four species present a 2n = 22 karyotype, composed by metacentric and submetacentric chromosomes, which were indistinguishable either after conventional staining or banding techniques. Constitutive heterochromatin was predominantly located at pericentromeric regions, and telomeric sequences (TTAGGG)n were restricted to the end of all chromosomes. Silver staining revealed Ag-NORs located at the short arm of pair 7, and heteromorphism in size of NOR signals was also observed. By contrast, FISH with ribosomal probes clearly demonstrated absence of any heteromorphism in size of rDNA sequences, suggesting that the difference observed after Ag-staining should be attributed to differences in chromosomal condensation and/or gene activity rather than to the number of ribosomal cistrons. (AU)

FAPESP's process: 99/11653-6 - Evolutionary studies in vertebrates: I. Cytogenetic, molecular and morphological analyses. II. Karyotypical survey of Brazilian fauna. III. Cell and tissue collection of mammals, lizards and amphibians
Grantee:Yatiyo Yonenaga-Yassuda
Support Opportunities: Research Projects - Thematic Grants