Ribosomal DNA Nanoprobes studied by Fourier Transform Infrared spectroscopy

Author(s)

Fagundes, Jaciara ^[1] ; Castilho, Maiara L. ^[1] ; Tellez Soto, Claudio A. ^[1] ; Vieira, Lais de Souza ^[1] ; Canevari, Renata A. ^[1] ; Favero, Priscila P. ^[1] ; Martin, Airton A. ^[1] ; Raniero, Leandro ^[1]

Affiliation

^[1] Univ Vale do Paraiba UniVap, Inst Res & Dev, BR-12244000 Sao Paulo - Brazil

• Document type: Journal article
• Source: SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY; v. 118, p. 28-35, JAN 24 2014.
• Web of Science Citations: 5

Abstract

Paracoccidioides brasiliensis (P. brasiliensis) is a thermo-dimorphic fungus that causes paracoccidioidomycosis. Brazil epidemiological data shows that endemic areas are the subtropical regions, especially where agricultural activities predominate such as the Southeast, South, and Midwest. There are several tests to diagnose paracoccidioidomycosis, but they have many limitations such as low sensitivity, high cost, and a cross-reacting problem. In this work, gold nanoprobes were used to identify P. brasiliensis as an alternative diagnostic technique, which is easier to apply, costs less, and has great potential for application. The specific Ribosomal sequence of P. brasiliensis DNA was amplified and used to design the nanoprobes using a thiol-modified oligonucleotide. The results of positive and negative tests were done by UV-visible and Fourier Transform Infrared (FT-IR) measurements. The deconvolution of FT-IR sample spectra showed differences in the vibrational modes from the hydrogen bridge NHN and NHO bands that form the double helix DNA for samples matching the DNA sequence of nanoprobes that could be used to classify the samples. (C) 2013 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 09/09559-5 - Gold nano-probe for Paracoccidioides brasiliensis diagnoses
• Grantee: Leandro José Raniero
• Support Opportunities: Regular Research Grants