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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Initial hemodynamic and metabolic effects of intestinal hypothermic perfusion in situ: an alternative model of canine intestinal autotransplantation

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Ruy Jorge Cruz Junior ; Cristiano de Jesus Correia ; Luiz Francisco Poli de Figueiredo ; Maurício Rocha e Silva
Total Authors: 4
Document type: Journal article
Source: Acta Cirurgica Brasileira; v. 19, n. 4, p. 383-392, Ago. 2004.

Intestinal transplantation is an acceptable therapy for children and adults with short bowel syndrome. The great majority of large animal experimental models of intestinal transplantation are complex and take a lot of time to be performed. In this study, we developed an alternative model of intestinal autotransplantation and evaluate the initial impact of isolated hypothermic intestinal perfusion with Ringer’s lactate solution on hemodynamic and metabolic parameters. METHODS: Six pentobarbital anesthetized mongrel dogs were used in this study (22,8±1,4 Kg). Systemic hemodynamic were evaluated through a Swan-Ganz and arterial catheters; while gastrointestinal tract perfusion by superior mesenteric vein blood flow (SMVBF, ultrasonic flowprobe) and intestinal mucosal pCO2 (pCO2-int and pCO2-gap, gas tonometry). Initially, the proximal jejunum and distal ileum were transected; at the basis of the mesentery excepting the superior mesenteric artery and vein. The small bowel was then perfused in situ with cold (4ºC) Ringer’s lactate solution for 30 minutes, with an automatic pump. The animals where observed for 120 minutes after reperfusion. Blood samples were collected from thoracic aorta for gas blood analysis. RESULTS: Hypothermic intestinal perfusion induced a partial reduction on SMVBF, only in the first 30 min of reperfusion (398±102,8 to 587±70,9 ml/min) and an increase on pCO2-gap (2±2,7 to 29,8±6 mmHg). During the experimental protocol, we did not observe significant alterations on systemic hemodynamic or metabolic parameters (MAP, CO, pH, base excess and hemoglobin levels) as well as on central core temperature. CONCLUSION: The model of intestinal transplantation is very useful to test different preservation solutions and/or antioxidants drugs, which are often used in clinical small bowel transplantation. (AU)

FAPESP's process: 98/15658-0 - Initial treatment of shock
Grantee:Maurício Rocha e Silva
Support type: Research Projects - Thematic Grants