Exercise training improves muscle vasodilatation in individuals with T786C polymorphism of endothelial nitric oxide synthase gene

Author(s):Show less -

Negrao, Marcelo V. ; Alves, Cleber R. ; Alves, Guilherme B. ; Pereira, Alexandre C. ; Dias, Rodrigo G. ; Laterza, Mateus C. ; Mota, Gloria F. ^[1] ; Oliveira, Edilamar M. ^[1] ; Bassaneze, Vinicius ; Krieger, Jose E. ; Negrao, Carlos E. ^{[1, 2]} ; Rondon, Maria Urbana P. B.

Affiliation

^[1] Univ Sao Paulo, Sch Phys Educ & Sport, BR-05403000 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Sch Med, Inst Coracao InCor, Unidade Reabilitacao Cardiovasc & Fisiol Exercici, BR-05403000 Sao Paulo - Brazil

• Document type: Journal article
• Source: Physiological Genomics; v. 42A, n. 1, p. 71-77, SEP 2010.
• Web of Science Citations: 15

Abstract

Negrao MV, Alves CR, Alves GB, Pereira AC, Dias RG, Laterza MC, Mota GF, Oliveira EM, Bassaneze V, Krieger JE, Negrao CE, Rondon MU. Exercise training improves muscle vasodilatation in individuals with T786C polymorphism of endothelial nitric oxide synthase gene. Physiol Genomics 42A: 71-77, 2010. First published July 6, 2010; doi:10.1152/physiolgenomics.00145.2009.-Allele T at promoter region of the eNOS gene has been associated with an increase in coronary disease mortality, suggesting that this allele increases susceptibility for endothelial dysfunction. In contrast, exercise training improves endothelial function. Thus, we hypothesized that: 1) Muscle vasodilatation during exercise is attenuated in individuals homozygous for allele T, and 2) Exercise training improves muscle vasodilatation in response to exercise for TT genotype individuals. From 133 preselected healthy individuals genotyped for the T786C polymorphism, 72 participated in the study: TT (n = 37; age 27 +/- 1 yr) and CT + CC (n = 35; age 26 +/- 1 yr). Forearm blood flow (venous occlusion plethysmography) and blood pressure (oscillometric automatic cuff) were evaluated at rest and during 30% handgrip exercise. Exercise training consisted of three sessions per week for 18 wk, with intensity between anaerobic threshold and respiratory compensation point. Resting forearm vascular conductance (FVC, P = 0.17) and mean blood pressure (P = 0.70) were similar between groups. However, FVC responses during handgrip exercise were significantly lower in TT individuals compared with CT + CC individuals (0.39 +/- 0.12 vs. 1.08 +/- 0.27 units, P = 0.01). Exercise training significantly increased peak VO(2) in both groups, but resting FVC remained unchanged. This intervention significantly increased FVC response to handgrip exercise in TT individuals (P = 0.03), but not in CT + CC individuals (P = 0.49), leading to an equivalent FVC response between TT and CT + CC individuals (1.05 +/- 0.18 vs. 1.59 +/- 0.27 units, P = 0.27). In conclusion, exercise training improves muscle vasodilatation in response to exercise in TT genotype individuals, demonstrating that genetic variants influence the effects of interventions such as exercise training. (AU)

• FAPESP's process: 05/59740-7 - Physical exercise and autonomic control in cardiovascular physiopathology
• Grantee: Carlos Eduardo Negrão
• Support type: Research Projects - Thematic Grants