Changes in gene expression and methylation in the blood of patients with first-episode psychosis

Autor(es):Mostrar menos -

Ota, Vanessa Kiyomi ^{[1, 2]} ; Noto, Cristiano ^{[3, 4, 2]} ; Gadelha, Ary ^{[4, 2]} ; Santoro, Marcos Leite ^{[1, 2]} ; Spindola, Leticia Maria ^{[1, 2]} ; Gouvea, Eduardo Sauerbronn ^{[3, 4]} ; Stilhano, Roberta Sessa ^[5] ; Ortiz, Bruno Bertolucci ^[4] ; Silva, Patricia Natalia ^{[1, 4, 2]} ; Sato, Joao Ricardo ^{[2, 6]} ; Han, Sang Won ^[5] ; Cordeiro, Quirino ^{[3, 4]} ; Bressan, Rodrigo Affonseca ^{[4, 2]} ; Belangero, Sintia Iole ^{[1, 4, 2]}

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo UNIFESP, Dept Morphol & Genet, Div Genet, Sao Paulo - Brazil

^[2] Univ Fed Sao Paulo, LiNC Interdisciplinary Lab Clin Neurosci, BR-04023900 Sao Paulo - Brazil

^[3] ISCMSP, Dept Psychiat, Sao Paulo - Brazil

^[4] Univ Fed Sao Paulo, Dept Psychiat, BR-04023900 Sao Paulo - Brazil

^[5] Univ Fed Sao Paulo, Dept Biophys, BR-04023900 Sao Paulo - Brazil

^[6] Univ Fed ABC, Ctr Math Computat & Cognit, Santo Andre, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SCHIZOPHRENIA RESEARCH; v. 159, n. 2-3, p. 358-364, NOV 2014.
• Citações Web of Science: 22

Resumo

Schizophrenia is a severe mental health disorder with high heritability. The investigation of individuals during their first-episode psychosis (FEP), before the progression of psychotic disorders and especially before treatment with antipsychotic medications, is particularly helpful for understanding this complex disease and for the identification of potential biomarkers. In this study, we compared the expression of genes that are involved in neurotransmission and neurodevelopment of antipsychotic-naive FEP in the peripheral blood of patients (n=51) and healthy controls (n=51). In addition, we investigated the differentially expressed genes with respect to a) DNA methylation, b) the correlation between gene expression and clinical variables (PANSS), and c) gene expression changes after risperidone treatment. Expression levels of 11 genes were quantified with SYBR Green. For methylation analysis, bisulfite sequencing was performed. A significant decrease in GCH1 mRNA levels was observed in FEP patients relative to controls. Also, when we compare the FEP patients after risperidone treatment with controls, this difference remains significant, and no significant differences were observed in GCH1 mRNA levels when comparing patients before and after risperidone treatment. Additionally, although the differences were non-significant after Bonferroni correction, the expression of GCH1 seemed to be correlated with PANSS scores, and the GCH1 promoter region was more methylated in FEP than in controls, thus corroborating the results obtained at the mRNA level. Few studies have been conducted on GCH1, and future studies are needed to clarify its potential role in the progression of schizophrenia. (C) 2014 Elsevier B. V. All rights reserved. (AU)

• Processo FAPESP: 10/08968-6 - Investigação de marcadores genéticos e epigenéticos: uma abordagem translacional para o tratamento de esquizofrenia
• Beneficiário: Síntia Iole Nogueira Belangero
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/50740-5 - Prevenção na esquizofrenia e no transtorno bipolar da neurociência à comunidade: uma plataforma multifásica, multimodal e translacional para investigação e intervenção
• Beneficiário: Rodrigo Affonseca Bressan
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 13/10498-6 - Aprendizado de máquina em neuroimagem: desenvolvimento de métodos e aplicações clínicas em transtornos psiquiátricos
• Beneficiário: João Ricardo Sato
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 10/19176-3 - Análise de expressão gênica e metilação de DNA de pacientes em primeiro episódio psicótico virgens de tratamento
• Beneficiário: Vanessa Kiyomi Ota Kuniyoshi
• Modalidade de apoio: Bolsas no Brasil - Doutorado