Saturated Fatty Acids Modulate Autophagy's Proteins in the Hypothalamus

Autor(es):Mostrar menos -

Portovedo, Mariana ^{[1, 2]} ; Ignacio-Souza, Leticia M. ^[3] ; Bombassaro, Bruna ^[1] ; Coope, Andressa ^[1] ; Reginato, Andressa ^{[1, 2]} ; Razolli, Daniela S. ^[1] ; Torsoni, Marcio A. ^{[1, 2]} ; Torsoni, Adriana S. ^{[2, 1]} ; Leal, Raquel F. ^[1] ; Velloso, Licio A. ^[1] ; Milanski, Marciane ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, UNICAMP, Obes & Comorbid Res Ctr, Campinas, SP - Brazil

^[2] Univ Estadual Campinas, UNICAMP, Fac Sci Appl, Limeira - Brazil

^[3] Univ Fed Mato Grosso, Fac Nutr, Cuiaba - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 10, n. 3 MAR 18 2015.
• Citações Web of Science: 23

Resumo

Autophagy is an important process that regulates cellular homeostasis by degrading dysfunctional proteins, organelles and lipids. In this study, the hypothesis that obesity could lead to impairment in hypothalamic autophagy in mice was evaluated by examining the hypothalamic distribution and content of autophagic proteins in animal with obesity induced by 8 or 16 weeks high fat diet to induce obesity and in response to intracerebroventricular injections of palmitic acid. The results showed that chronic exposure to a high fat diet leads to an increased expression of inflammatory markers and downregulation of autophagic proteins. In obese mice, autophagic induction leads to the downregulation of proteins, such as JNK and Bax, which are involved in the stress pathways. In neuron cell-line, palmitate has a direct effect on autophagy even without inflammatory activity. Understanding the cellular and molecular bases of overnutrition is essential for identifying new diagnostic and therapeutic targets for obesity. (AU)

• Processo FAPESP: 11/14565-4 - Caracterização da autofagia em hipotálamo de diferentes modelos animais de obesidade
• Beneficiário: Mariana Portovedo de Oliveira Araújo
• Linha de fomento: Bolsas no Brasil - Mestrado
• Processo FAPESP: 11/51205-6 - Papel da autofagia na indução de apoptose em neurônios hipotalâmicos em resposta a ácidos graxos saturados
• Beneficiário: Marciane Milanski Ferreira
• Linha de fomento: Auxílio à Pesquisa - Regular