Glyceraldehyde 3-Phosphate Dehydrogenase-Telomere Association Correlates with Redox Status in Trypanosoma cruzi

Autor(es)

Pariona-Llanos, Ricardo ^{[1, 2]} ; Pavani, Raphael Souza ^{[1, 2]} ; Reis, Marcelo ^{[1, 2]} ; Noel, Vincent ^{[1, 2]} ; Silber, Ariel Mariano ^[3] ; Armelin, Hugo Aguirre ^{[4, 1, 2]} ; Cano, Maria Isabel Nogueira ^[5] ; Elias, Maria Carolina ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Inst Butantan, Lab Especial Ciclo Celular, Sao Paulo - Brazil

^[2] Inst Butantan, Ctr Toxins Immune Response & Cell Signaling CeTIC, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Inst Ciencias Biomed, Dept Parasitol, Unit Drug Discovery, BR-05508 Sao Paulo - Brazil

^[4] Univ Sao Paulo, Inst Quim, Dept Bioquim, BR-01498 Sao Paulo - Brazil

^[5] Univ Estadual Paulista Julio de Mesquita Filho UN, Inst Biociencias, Dept Genet, Botucatu, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 10, n. 3 MAR 16 2015.
• Citações Web of Science: 12

Resumo

Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is a classical metabolic enzyme involved in energy production and plays a role in additional nuclear functions, including transcriptional control, recognition of misincorporated nucleotides in DNA and maintenance of telomere structure. Here, we show that the recombinant protein T. cruzi GAPDH (rTcGAPDH) binds single-stranded telomeric DNA. We demonstrate that the binding of GAPDH to telomeric DNA correlates with the balance between oxidized and reduced forms of nicotinamide adenine dinucleotides (NAD+/NADH). We observed that GAPDH-telomere association and NAD+/NADH balance changed throughout the T. cruzi life cycle. For example, in replicative epimastigote forms of T. cruzi, which show similar intracellular concentrations of NAD+ and NADH, GAPDH binds to telomeric DNA in vivo and this binding activity is inhibited by exogenous NAD+. In contrast, in the T. cruzi non-proliferative trypomastigote forms, which show higher NAD+ concentration, GAPDH was absent from telomeres. In addition, NAD+ abolishes physical interaction between recombinant GAPDH and synthetic telomere oligonucleotide in a cell free system, mimicking exogenous NAD+ that reduces GAPDH-telomere interaction in vivo. We propose that the balance in the NAD+/NADH ratio during T. cruzi life cycle homeostatically regulates GAPDH telomere association, suggesting that in trypanosomes redox status locally modulates GAPDH association with telomeric DNA. (AU)

• Processo FAPESP: 13/07467-1 - CeTICS - Centro de Toxinas, Imuno-Resposta e Sinalização Celular
• Beneficiário: Hugo Aguirre Armelin
• Linha de fomento: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 11/50631-1 - Metabolismo de histidina: o papel da via histina-glutamato na biologia do Trypanosoma cruzi
• Beneficiário: Ariel Mariano Silber
• Linha de fomento: Auxílio à Pesquisa - Regular
• Processo FAPESP: 05/00154-1 - Identificação das origens e da maquinária de replicação em células de Trypanosoma
• Beneficiário: Maria Carolina Quartim Barbosa Elias Sabbaga
• Linha de fomento: Auxílio à Pesquisa - Apoio a Jovens Pesquisadores