Dynamic changes in the spatio-temporal expression of the beta-defensin SPAG11C in the developing rat epididymis and its regulation by androgens

Autor(es)

Ribeiro, Camilla M. ^[1] ; Queiroz, Daniel B. C. ^[1] ; Patrao, Marilia T. C. C. ^[1] ; Denadai-Souza, Alexandre ^[1] ; Romano, Renata M. ^[1] ; Silva, Erick J. R. ^[1] ; Avellar, Maria Christina W. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Escola Paulista Med, Dept Pharmacol, Sect Expt Endocrinol, BR-04044020 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Molecular and Cellular Endocrinology; v. 404, n. C, p. 141-150, MAR 15 2015.
• Citações Web of Science: 5

Resumo

Herein, we characterized the spatio-temporal expression, cellular distribution and regulation by androgens of the P-defensin SPAG11C, the rat ortholog of the human SPAG11B isoform C, in the developing epididymis by using RT-PCR, in situ hybridization and immunohistochemistry. We observed that Spag11c mRNA was ubiquitously expressed in rat fetuses, but preferentially detected in male reproductive tissues at adulthood. SPAG11C (mRNA and protein) was prenatally mainly detected in the mesenchyme of the Wolffian duct, switching gradually after birth to a predominant localization in the epididymis epithelium during postnatal development. In the adult epididymis, smooth muscle and interstitial cells were also identified as sources of SPAG11C. Furthermore, SPAG11C was differentially immunolocalized on spermatozoa surface during their transit from testis throughout caput and cauda epididymis. Developmental and surgical castration studies suggested that androgens contribute to the epididymal cell type- and region-specific modulation of SPAG11C mRNA levels and immunolocalization. Together our findings provide novel insights into the potential role of beta-defensins in the epididymis. (C) 2015 Elsevier Ireland Ltd. All rights reserved. (AU)

• Processo FAPESP: 10/52711-0 - Aspectos funcionais da proteina spag11, papel fisiologico e mecanismo de acao.
• Beneficiário: Maria Christina Werneck de Avellar
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 09/14649-3 - Caracterização de isoformas do gene antimicrobiano Spag11 e suas proteínas de interação no desenvolvimento do rato: regulação da expressão e potenciais papéis fisiológicos do gene Spag11
• Beneficiário: Camilla Moreira Ribeiro
• Modalidade de apoio: Bolsas no Brasil - Doutorado