Lycopene-rich tomato oleoresin modulates plasma adiponectin concentration and mRNA levels of adiponectin, SIRT1, and FoxO1 in adipose tissue of obese rats

Autor(es)

Luvizotto, R. A. M. ^{[1, 2]} ; Nascimento, A. F. ^[2] ; Miranda, N. C. M. ^[1] ; Wang, X-D ^[3] ; Ferreira, A. L. A. ^[1]

Afiliação do(s) autor(es)

^[1] Botucatu Med Sch UNESP, Dept Internal Med, Sao Paulo - Brazil

^[2] Fed Univ Mato Grosso UFMT, Inst Hlth Sci, BR-78557267 Sinop, MG - Brazil

^[3] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 - USA

• Tipo de documento: Artigo Científico
• Fonte: HUMAN & EXPERIMENTAL TOXICOLOGY; v. 34, n. 6, p. 612-619, JUN 2015.
• Citações Web of Science: 14

Resumo

Aim: To investigate whether lycopene can modulate adiponectin levels and SIRT1 and FoxO1 gene expression in the adipose tissue of diet-induced obese rats. Methods: Male Wistar rats were first fed with hypercaloric diet (HD, n = 12) for 6 weeks, and afterward, these rats were randomly assigned to receive HD (n = 6) or HD with lycopene-rich tomato oleoresin (equivalent to lycopene 10 mg/kg body weight (BW)/day, HD + L, n = 6) by gavage for additional 6 weeks. Plasma lycopene and adiponectin levels were analyzed by high-performance liquid chromatography and immunoassay, respectively. The messenger RNA (mRNA) expressions of adiponectin, Sirtuin 1 (SIRT1), Forkhead box O 1 (FoxO1), fatty acid translocase/cluster of differentiation 36 (FAT/CD36), and PPAR in adipose tissues were determined by quantitative polymerase chain reaction. Results: Lycopene was detected in the plasma of rats in HD + L group but not in the HD group. Although both BW and adiposity were not different between the two groups, there was a significant increase in both plasma concentration and mRNA expression of adiponectin in the adipose tissue of the HD + L group. In addition, the lycopene supplementation upregulated mRNA expressions of SIRT1, FoxO1, and FAT/CD36 but downregulated PPAR in adipose tissue of obese rats. Conclusion: These data suggest that lycopene, in the concentration used, is not toxic and also its health benefits in adipose tissue may play a role against obesity-related complications. (AU)

• Processo FAPESP: 11/22786-0 - Influência do licopeno sobre marcadores de estresse oxidativo no tecido adiposo de ratos obesos
• Beneficiário: Renata de Azevedo Melo Luvizotto Nascimento
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado
• Processo FAPESP: 11/19934-8 - Influência do licopeno sobre os níveis de adiponectina no tecido adiposo de animais submetidos à dieta hipercalórica
• Beneficiário: Natália Carla Martins Miranda
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 10/19746-4 - Efeito da suplementação com licopeno em modelo experimental de obesidade induzida por dieta
• Beneficiário: Renata de Azevedo Melo Luvizotto Nascimento
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 10/06100-9 - Efeito da suplementação com licopeno na obesidade em modelo experimental com ratos Zucker
• Beneficiário: Ana Lucia do Anjos Ferreira
• Modalidade de apoio: Auxílio à Pesquisa - Regular