Microspheres prepared with different co-polymers of poly(lactic-glycolic acid) (PLGA) or with chitosan cause distinct effects on macrophages

Autor(es)

Bitencourt, Claudia da Silva ^{[1, 2]} ; da Silva, Leticia Bueno ^[2] ; Tartari Pereira, Priscilla Aparecida ^[2] ; Gelfuso, Guilherme Martins ^[3] ; Faccioli, Lucia Helena ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Ctr Univ Fac Associadas Ensino UNIFAE, BR-13870377 Sao Joao Da Boa Vista, SP - Brazil

^[2] Univ Sao Paulo, Fac Ciancias Farmaceut Ribeirao Preto, Dept Anal Clin Toxicol & Bromatol, BR-14040903 Ribeirao Preto, SP - Brazil

^[3] Univ Brasilia, Fac Ciencias Saude, LTMAC, BR-70910900 Brasilia, DF - Brazil

• Tipo de documento: Artigo Científico
• Fonte: COLLOIDS AND SURFACES B-BIOINTERFACES; v. 136, p. 678-686, DEC 1 2015.
• Citações Web of Science: 10

Resumo

Microencapsulation of bioactive molecules for modulating the immune response during infectious or inflammatory events is a promising approach, since microspheres (MS) protect these labile biomolecules against fast degradation, prolong the delivery over longer periods of time and, in many situations, target their delivery to site of action, avoiding toxic side effects. Little is known, however, about the influence of different polymers used to prepare MS on macrophages. This paper aims to address this issue by evaluating in vitro cytotoxicity, phagocytosis profile and cytokines release from alveolar macrophages (J-774.1) treated with MS prepared with chitosan, and four different co-polymers of PLGA {[}poly (lactic-co-glycolic acid)]. The five MS prepared presented similar diameter and zeta potential each other. Chitosan-MS showed to be cytotoxic to J-774.1 cells, in contrast to PLGA-MS, which were all innocuous to this cell linage. PLGA 5000-MS was more efficiently phagocytized by macrophages compared to the other MS tested. PLGA 5000-MS and 5002-MS induced significant production of TNF-alpha, while 5000-MS, 5004-MS and 7502-MS decreased spontaneous IL-6 release. Nevertheless, only ALGA 5002-MS induced significant NFkB/SEAP activation. These findings together show that MS prepared with distinct PLGA co-polymers are differently recognized by macrophages, depending on proportion of lactic and glycolic acid in polymeric chain, and on molecular weight of the co-polymer used. Selection of the most adequate polymer to prepare a microparticulate drug delivery system to modulate immunologic system may take into account, therefore, which kind of immunomodulatory response is more adequate for the required treatment. (C) 2015 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 07/04741-4 - Estudo da atividade de hialuronidases solúveis ou encapsuladas em associação ou não com MK886
• Beneficiário: Claudia da Silva Bitencourt
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 02/12856-2 - Modulação das respostas imunes inata e adquirida por leucotrienos e prostaglandinas
• Beneficiário: Lúcia Helena Faccioli
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 09/07169-5 - Mediadores lipídicos como reguladores da resposta imune
• Beneficiário: Lúcia Helena Faccioli
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 05/54855-0 - Toxinas animais: estrutura, função e aplicações biotecnológicas
• Beneficiário: Suely Vilela
• Modalidade de apoio: Auxílio à Pesquisa - Temático