Microspheres prepared with different co-polymers of poly(lactic-glycolic acid) (PLGA) or with chitosan cause distinct effects on macrophages

Author(s)

Bitencourt, Claudia da Silva ^{[1, 2]} ; da Silva, Leticia Bueno ^[2] ; Tartari Pereira, Priscilla Aparecida ^[2] ; Gelfuso, Guilherme Martins ^[3] ; Faccioli, Lucia Helena ^{[1, 2]}

Affiliation

^[1] Ctr Univ Fac Associadas Ensino UNIFAE, BR-13870377 Sao Joao Da Boa Vista, SP - Brazil

^[2] Univ Sao Paulo, Fac Ciancias Farmaceut Ribeirao Preto, Dept Anal Clin Toxicol & Bromatol, BR-14040903 Ribeirao Preto, SP - Brazil

^[3] Univ Brasilia, Fac Ciencias Saude, LTMAC, BR-70910900 Brasilia, DF - Brazil

• Document type: Journal article
• Source: COLLOIDS AND SURFACES B-BIOINTERFACES; v. 136, p. 678-686, DEC 1 2015.
• Web of Science Citations: 10

Abstract

Microencapsulation of bioactive molecules for modulating the immune response during infectious or inflammatory events is a promising approach, since microspheres (MS) protect these labile biomolecules against fast degradation, prolong the delivery over longer periods of time and, in many situations, target their delivery to site of action, avoiding toxic side effects. Little is known, however, about the influence of different polymers used to prepare MS on macrophages. This paper aims to address this issue by evaluating in vitro cytotoxicity, phagocytosis profile and cytokines release from alveolar macrophages (J-774.1) treated with MS prepared with chitosan, and four different co-polymers of PLGA {[}poly (lactic-co-glycolic acid)]. The five MS prepared presented similar diameter and zeta potential each other. Chitosan-MS showed to be cytotoxic to J-774.1 cells, in contrast to PLGA-MS, which were all innocuous to this cell linage. PLGA 5000-MS was more efficiently phagocytized by macrophages compared to the other MS tested. PLGA 5000-MS and 5002-MS induced significant production of TNF-alpha, while 5000-MS, 5004-MS and 7502-MS decreased spontaneous IL-6 release. Nevertheless, only ALGA 5002-MS induced significant NFkB/SEAP activation. These findings together show that MS prepared with distinct PLGA co-polymers are differently recognized by macrophages, depending on proportion of lactic and glycolic acid in polymeric chain, and on molecular weight of the co-polymer used. Selection of the most adequate polymer to prepare a microparticulate drug delivery system to modulate immunologic system may take into account, therefore, which kind of immunomodulatory response is more adequate for the required treatment. (C) 2015 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 07/04741-4 - Study of activity of soluble hyaluronidases or encapsulated in association or not with MK886
• Grantee: Claudia da Silva Bitencourt
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 02/12856-2 - Modulation of innate and acquired immune responses by leukotrienes and prostaglandins
• Grantee: Lúcia Helena Faccioli
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 09/07169-5 - Lipid mediators as regulators of immune response
• Grantee: Lúcia Helena Faccioli
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 05/54855-0 - Animal toxins: structure, function and biotechnological applications
• Grantee: Suely Vilela
• Support Opportunities: Research Projects - Thematic Grants