Consequence of hyperhomocysteinaemia on (1)-adrenoceptor-mediated contraction in the rat corpus cavernosum: the role of reactive oxygen species

Autor(es)

Coco, Hariane ^[1] ; Pernomian, Larissa ^[2] ; Marchi, Katia C. ^[1] ; Gomes, Mayara S. ^[2] ; de Andrade, Claudia R. ^[3] ; Ramalho, Leandra N. Z. ^[4] ; Tirapelli, Carlos R. ^[5] ; de Oliveira, Ana M. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Pharmacol, BR-14040903 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Lab Vasc Injury, Ave Cafe W-S, BR-14040903 Ribeirao Preto, SP - Brazil

^[3] Fac Inta, Nucleus Appl Bioprospect & Mol Res, Fortaleza, Ceara - Brazil

^[4] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Pathol & Legal Med, BR-14040903 Ribeirao Preto, SP - Brazil

^[5] Univ Sao Paulo, Escola Enfermagem Ribeirao Preto, Pharmacol Lab, BR-14040903 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Pharmacy and Pharmacology; v. 68, n. 1, p. 63-75, JAN 2016.
• Citações Web of Science: 4

Resumo

ObjectivesOur main objective was to investigate the mechanisms underlying the effects of hyperhomocysteinaemia (HHcy) on contractile response mediated by (1)-adrenoceptors in the rat corpus cavernosum. MethodsConcentration-response curves for phenylephrine (PE) were obtained in strips of corpus cavernosum, in absence or after incubation with tiron, tempol or polyethylene glycol (PEG)-catalase combined or not with tempol. We also measured the superoxide anion (O-2(-)) and hydrogen peroxide (H2O2) generation, superoxide dismutase (SOD) and catalase activity and -actin expression in rat corpus cavernosum from both groups. Key findingsHHcy increased PE-induced contraction in cavernosal strips. Tiron, PEG-catalase or tempol increased PE-induced contraction in strips from control rats, but it was not altered by tiron or PEG-catalase in HHcy rats, whereas tempol reduced this response. The combination of PEG-catalase and tempol did not alter the contractile response to PE in both groups. HHcy increased O-2(-) generation and SOD activity, whereas H2O2 concentration was reduced. Finally, HHcy did not alter catalase activity or expression of -actin. ConclusionsThe major new finding from this study is that HHcy induced a marked increase in PE-induced contraction in rat corpus cavernosum by a mechanism that involves increased O-2(-) generation and it could play a role in the pathogenesis of erectile dysfunction associated with HHcy. (AU)

• Processo FAPESP: 10/03614-1 - Consequências da Hiperhomocisteinemia no sistema endotelina em corpo cavernoso de ratos
• Beneficiário: Hariane Côco
• Modalidade de apoio: Bolsas no Brasil - Mestrado