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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Consequence of hyperhomocysteinaemia on (1)-adrenoceptor-mediated contraction in the rat corpus cavernosum: the role of reactive oxygen species

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Author(s):
Coco, Hariane [1] ; Pernomian, Larissa [2] ; Marchi, Katia C. [1] ; Gomes, Mayara S. [2] ; de Andrade, Claudia R. [3] ; Ramalho, Leandra N. Z. [4] ; Tirapelli, Carlos R. [5] ; de Oliveira, Ana M. [2]
Total Authors: 8
Affiliation:
[1] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Pharmacol, BR-14040903 Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Lab Vasc Injury, Ave Cafe W-S, BR-14040903 Ribeirao Preto, SP - Brazil
[3] Fac Inta, Nucleus Appl Bioprospect & Mol Res, Fortaleza, Ceara - Brazil
[4] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Pathol & Legal Med, BR-14040903 Ribeirao Preto, SP - Brazil
[5] Univ Sao Paulo, Escola Enfermagem Ribeirao Preto, Pharmacol Lab, BR-14040903 Ribeirao Preto, SP - Brazil
Total Affiliations: 5
Document type: Journal article
Source: Journal of Pharmacy and Pharmacology; v. 68, n. 1, p. 63-75, JAN 2016.
Web of Science Citations: 4
Abstract

ObjectivesOur main objective was to investigate the mechanisms underlying the effects of hyperhomocysteinaemia (HHcy) on contractile response mediated by (1)-adrenoceptors in the rat corpus cavernosum. MethodsConcentration-response curves for phenylephrine (PE) were obtained in strips of corpus cavernosum, in absence or after incubation with tiron, tempol or polyethylene glycol (PEG)-catalase combined or not with tempol. We also measured the superoxide anion (O-2(-)) and hydrogen peroxide (H2O2) generation, superoxide dismutase (SOD) and catalase activity and -actin expression in rat corpus cavernosum from both groups. Key findingsHHcy increased PE-induced contraction in cavernosal strips. Tiron, PEG-catalase or tempol increased PE-induced contraction in strips from control rats, but it was not altered by tiron or PEG-catalase in HHcy rats, whereas tempol reduced this response. The combination of PEG-catalase and tempol did not alter the contractile response to PE in both groups. HHcy increased O-2(-) generation and SOD activity, whereas H2O2 concentration was reduced. Finally, HHcy did not alter catalase activity or expression of -actin. ConclusionsThe major new finding from this study is that HHcy induced a marked increase in PE-induced contraction in rat corpus cavernosum by a mechanism that involves increased O-2(-) generation and it could play a role in the pathogenesis of erectile dysfunction associated with HHcy. (AU)

FAPESP's process: 10/03614-1 - Effects of the Hyperhomocysteinemia on the endothelin system in corpus cavernosum of rats
Grantee:Hariane Côco
Support Opportunities: Scholarships in Brazil - Master