Cachexia-associated adipose tissue morphological rearrangement in gastrointestinal cancer patients

Autor(es)

Batista, Jr., Miguel L. ^{[1, 2]} ; Henriques, Felipe S. ^[1] ; Neves, Rodrigo X. ^{[2, 1]} ; Olivan, Mireia R. ^[2] ; Matos-Neto, Emidio M. ^[2] ; Alcantara, Paulo S. M. ^[3] ; Maximiano, Linda F. ^[3] ; Otoch, Jose P. ^[3] ; Alves, Michele J. ^[2] ; Seelaender, Marilia ^[2]

Afiliação do(s) autor(es)

^[1] Univ Mogi das Cruzes, Integrated Grp Biotechnol, Lab Adipose Tissue Biol, Mogi Das Cruzes - Brazil

^[2] Univ Sao Paulo, Inst Biomed Sci, Canc Metab Res Grp, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Univ Hosp, Dept Clin Surg, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF CACHEXIA SARCOPENIA AND MUSCLE; v. 7, n. 1, p. 37-47, MAR 2016.
• Citações Web of Science: 29

Resumo

Background and aimsCachexia is a syndrome characterized by marked involuntary loss of body weight. Recently, adipose tissue (AT) wasting has been shown to occur before the appearance of other classical cachexia markers. We investigated the composition and rearrangement of the extracellular matrix, adipocyte morphology and inflammation in the subcutaneous AT (scAT) pad of gastrointestinal cancer patients. MethodsSurgical biopsies for scAT were obtained from gastrointestinal cancer patients, who were signed up into the following groups: cancer cachexia (CC, n=11), weight-stable cancer (WSC, n=9) and weight-stable control (non-cancer) (control, n=7). The stable weight groups were considered as those with no important weight change during the last year and body mass index <25kg/m(2). Subcutaneous AT fibrosis was quantified and characterized by quantitative PCR, histological analysis and immunohistochemistry. ResultsThe degree of fibrosis and the distribution and collagen types (I and III) were different in WSC and CC patients. CC patients showed more pronounced fibrosis in comparison with WSC. Infiltrating macrophages surrounding adipocytes and CD3 Ly were found in the fibrotic areas of scAT. Subcutaneous AT fibrotic areas demonstrated increased monocyte chemotactic protein 1 (MCP-1) and Cluster of Differentiation (CD)68 gene expression in cancer patients. ConclusionsOur data indicate architectural modification consisting of fibrosis and inflammatory cell infiltration in scAT as induced by cachexia in gastrointestinal cancer patients. The latter was characterized by the presence of macrophages and lymphocytes, more evident in the fibrotic areas. In addition, increased MCP-1 and CD68 gene expression in scAT from cancer patients may indicate an important role of these markers in the early phases of cancer. (AU)

• Processo FAPESP: 12/50079-0 - Inflamação sistêmica em pacientes com caquexia associada ao câncer: mecanismos e estratégias terapêuticas, uma abordagem em medicina translacional
• Beneficiário: Marilia Cerqueira Leite Seelaender
• Linha de fomento: Auxílio à Pesquisa - Temático
• Processo FAPESP: 10/51078-1 - Bases moleculares da caquexia: adipogênese e remodelagem da matriz extracelular do tecido adiposo branco de pacientes com câncer gastrointestinal
• Beneficiário: Miguel Luiz Batista Junior
• Linha de fomento: Auxílio à Pesquisa - Apoio a Jovens Pesquisadores
• Processo FAPESP: 08/54091-9 - Regulação e síntese de adipocinas na caquexia associada ao câncer: possível interação entre PPAR-gama e NF kappaB
• Beneficiário: Marilia Cerqueira Leite Seelaender
• Linha de fomento: Auxílio à Pesquisa - Regular