Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Cachexia-associated adipose tissue morphological rearrangement in gastrointestinal cancer patients

Full text
Author(s):
Batista, Jr., Miguel L. [1, 2] ; Henriques, Felipe S. [2] ; Neves, Rodrigo X. [1, 2] ; Olivan, Mireia R. [1] ; Matos-Neto, Emidio M. [1] ; Alcantara, Paulo S. M. [3] ; Maximiano, Linda F. [3] ; Otoch, Jose P. [3] ; Alves, Michele J. [1] ; Seelaender, Marilia [1]
Total Authors: 10
Affiliation:
[1] Univ Sao Paulo, Inst Biomed Sci, Canc Metab Res Grp, Sao Paulo - Brazil
[2] Univ Mogi das Cruzes, Integrated Grp Biotechnol, Lab Adipose Tissue Biol, Mogi Das Cruzes - Brazil
[3] Univ Sao Paulo, Univ Hosp, Dept Clin Surg, Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: JOURNAL OF CACHEXIA SARCOPENIA AND MUSCLE; v. 7, n. 1, p. 37-47, MAR 2016.
Web of Science Citations: 29
Abstract

Background and aimsCachexia is a syndrome characterized by marked involuntary loss of body weight. Recently, adipose tissue (AT) wasting has been shown to occur before the appearance of other classical cachexia markers. We investigated the composition and rearrangement of the extracellular matrix, adipocyte morphology and inflammation in the subcutaneous AT (scAT) pad of gastrointestinal cancer patients. MethodsSurgical biopsies for scAT were obtained from gastrointestinal cancer patients, who were signed up into the following groups: cancer cachexia (CC, n=11), weight-stable cancer (WSC, n=9) and weight-stable control (non-cancer) (control, n=7). The stable weight groups were considered as those with no important weight change during the last year and body mass index <25kg/m(2). Subcutaneous AT fibrosis was quantified and characterized by quantitative PCR, histological analysis and immunohistochemistry. ResultsThe degree of fibrosis and the distribution and collagen types (I and III) were different in WSC and CC patients. CC patients showed more pronounced fibrosis in comparison with WSC. Infiltrating macrophages surrounding adipocytes and CD3 Ly were found in the fibrotic areas of scAT. Subcutaneous AT fibrotic areas demonstrated increased monocyte chemotactic protein 1 (MCP-1) and Cluster of Differentiation (CD)68 gene expression in cancer patients. ConclusionsOur data indicate architectural modification consisting of fibrosis and inflammatory cell infiltration in scAT as induced by cachexia in gastrointestinal cancer patients. The latter was characterized by the presence of macrophages and lymphocytes, more evident in the fibrotic areas. In addition, increased MCP-1 and CD68 gene expression in scAT from cancer patients may indicate an important role of these markers in the early phases of cancer. (AU)

FAPESP's process: 12/50079-0 - Systemic inflammation in cachectic cancer patients: mechanisms and therapeutical strategies, a translational medicine approach
Grantee:Marilia Cerqueira Leite Seelaender
Support Opportunities: Research Projects - Thematic Grants