Nitric oxide and fever: immune-to-brain signaling vs. thermogenesis in chicks

Autor(es)

Dantonio, Valter ^{[1, 2]} ; Batalhao, Marcelo E. ^[3] ; Fernandes, Marcia H. M. R. ^[4] ; Komegae, Evilin N. ^[5] ; Buqui, Gabriela A. ^[6] ; Lopes, Norberto P. ^[6] ; Gargaglioni, Luciane H. ^{[1, 2]} ; Carnio, Evelin C. ^[3] ; Steiner, Alexandre A. ^[5] ; Bicego, Kenia C. ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ, Dept Anim Morphol & Physiol, Coll Agr & Vet Sci, Sao Paulo - Brazil

^[2] Natl Inst Sci & Technol Comparat Physiol INCT Fis, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Nursing Sch Ribeirao Preto, Sao Paulo - Brazil

^[4] Sao Paulo State Univ, Dept Anim Sci, Coll Agr & Vet Sci, Sao Paulo - Brazil

^[5] Univ Sao Paulo, Inst Biomed Sci, Dept Immunol, Sao Paulo, SP - Brazil

^[6] Univ Sao Paulo, Fac Pharmaceut Sci Ribeirao Preto, Dept Chem & Phys, Nucleo Pesquisa Prod Nat & Sintet, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY; v. 310, n. 10, p. R896-R905, MAY 15 2016.
• Citações Web of Science: 3

Resumo

Nitric oxide (NO) plays a role in thermogenesis but does not mediate immune-to-brain febrigenic signaling in rats. There are suggestions of a different situation in birds, but the underlying evidence is not compelling. The present study was designed to clarify this matter in 5-day-old chicks challenged with a low or high dose of bacterial LPS. The lower LPS dose (2 mu g/kg im) induced fever at 3-5 h postinjection, whereas 100 mu g/kg im decreased core body temperature (T-c) (at 1 h) followed by fever (at 4 or 5 h). Plasma nitrate levels increased 4 h after LPS injection, but they were not correlated with the magnitude of fever. The NO synthase inhibitor (NG-nitro-L-arginine methyl ester, L-NAME; 50 mg/kg im) attenuated the fever induced by either dose of LPS and enhanced the magnitude of the Tc reduction induced by the high dose in chicks at 31-32 degrees C. These effects were associated with suppression of metabolic rate, at least in the case of the high LPS dose. Conversely, the effects of L-NAME on Tc disappeared in chicks maintained at 35-36 degrees C, suggesting that febrigenic signaling was essentially unaffected. Accordingly, the LPS-induced rise in the brain level of PGE(2) was not affected by L-NAME. Moreover, L-NAME augmented LPS-induced huddling, which is indicative of compensatory mechanisms to run fever in the face of attenuated thermogenesis. Therefore, as in rats, systemic inhibition of NO synthesis attenuates LPS-induced fever in chicks by affecting thermoeffector activity and not by interfering with immune-to-brain signaling. This may constitute a conserved effect of NO in endotherms. (AU)

• Processo FAPESP: 14/50265-3 - Metabolismo e distribuição de xenobióticos naturais e sintéticos: da compreensão dos processos reacionais à geração de imagens teciduais
• Beneficiário: Norberto Peporine Lopes
• Linha de fomento: Auxílio à Pesquisa - Programa BIOTA - Temático
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• Beneficiário: Kênia Cardoso Bícego
• Linha de fomento: Auxílio à Pesquisa - Regular
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• Beneficiário: Alexandre Alarcon Steiner
• Linha de fomento: Auxílio à Pesquisa - Apoio a Jovens Pesquisadores
• Processo FAPESP: 12/19966-0 - Padrão cardiorrespiratório e ciclo sono-vigília de ratos e ratas expostos à fluoxetina durante o período prenatal
• Beneficiário: Luciane Helena Gargaglioni Batalhão
• Linha de fomento: Auxílio à Pesquisa - Regular
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• Beneficiário: Evelin Capellari Cárnio
• Linha de fomento: Auxílio à Pesquisa - Regular