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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Online Extraction Coupled to Liquid Chromatography Analysis (OLELC): Eliminating Traditional Sample Preparation Steps in the Investigation of Solid Complex Matrices

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Autor(es):
Ferreira, Vinicius G. ; Leme, Gabriel M. ; Cavalheiro, Alberto J. ; Funari, Cristiano S.
Número total de Autores: 4
Tipo de documento: Artigo Científico
Fonte: Analytical Chemistry; v. 88, n. 17, p. 8421-8427, SEP 6 2016.
Citações Web of Science: 8
Resumo

Current methods employed for the analysis of the chemical composition of solid matrices (such as plant, animal, or human tissues; soil; etc.) often require many sample treatment steps, including an extraction step with exclusively dedicated solvents. This work describes an optimized analytical setup in which the extraction of a solid sample is directly coupled to its analysis by high-performance liquid chromatography. This approach avoids (i) the use of pumps and valves other than those comprising the HPLC instrument, (ii) the use of solvents other than those of the mobile phase, and (iii) the need to stop the mobile phase flow at any time during the full analytical procedure. The compatibility of this approach with the direct analysis of fresh tissues (leaves, stems, and seeds of four plant species with dissimilar chemical compositions) was successfully demonstrated, leading to the elimination of sample preparation steps such as drying, grinding, concentration, dilution, and filtration, among others. This work describes a new, simple, and efficient green approach to minimize or eliminate sample treatment procedures. It could be easily applied for quality control of plant materials and their derived products through chromatographic fingerprints and for untargeted metabolomic investigations of solid matrices, among other applications. (AU)

Processo FAPESP: 13/07600-3 - CIBFar - Centro de Inovação em Biodiversidade e Fármacos
Beneficiário:Glaucius Oliva
Linha de fomento: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
Processo FAPESP: 10/18840-7 - Cromatografia líquida verde (água, etanol e temperatura)aplicada ao estudo de plantas medicinais
Beneficiário:Cristiano Soleo de Funari
Linha de fomento: Bolsas no Brasil - Pós-Doutorado