Edema and Nociception Induced by Philodryas patagoniensis Venom in Mice: A Pharmacological Evaluation with Implications for the Accident Treatment

Autor(es)

Lopes, Priscila Hess ; Rocha, Marisa M. T. ; Kuniyoshi, Alexandre Kazuo ; Vieira Portaro, Fernanda Calheta ; Goncalves, Luis Roberto C.

• Tipo de documento: Artigo Científico
• Fonte: Journal of Pharmacology and Experimental Therapeutics; v. 361, n. 3, p. 349-354, JUN 1 2017.
• Citações Web of Science: 2

Resumo

We have investigated the mechanisms involved in the genesis of edema and nociception induced by Philodryas patagoniensis venom (PpV) injected into the footpad of mice. PpV induced dose-related edema and nociceptive effects. Pretreatment of mice with cyclooxygenase inhibitor (indomethacin), but not with cyclooxygenase 2 inhibitor (celecoxib) markedly inhibited both effects. Pretreatments with H-1 receptor antagonist (promethazine) or with dual histamine-serotonin inhibitor (cyproheptadine) failed in inhibiting both effects. In groups pretreated with captopril (angiotensin-converting enzyme inhibitor) the edema was unaltered, but nociception was clearly increased, suggesting the participation of kinins in the pathophysiology of the nociception but not of the edema-forming effect of PpV. When PpV was treated with EDTA, the nociception was similar to the one induced by untreated venom, but edema was markedly reduced. We concluded that PpV-induced edema and nociception have cyclooxygenase eicosanoids as the main mediators and no participation of vasoactive amines. Kinins seem to participate in nociception but not in edema induced by PpV. The results also suggest that metalloproteinases are the main compounds responsible for the edema, but not for the nociception induced by this venom. (AU)

• Processo FAPESP: 15/17053-5 - Estudo da ativação de inflamassomas, em queratinócitos humanos, por ação do veneno da aranha Loxosceles laeta e sua esfingomielinase D
• Beneficiário: Priscila Hess Lopes
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 13/15344-7 - Eficácia do soro antibotrópico produzido no Instituto Butantan: obtenção, caracterização e neutralização de serinopeptidases de interesse do veneno de Bothrops jararaca
• Beneficiário: Alexandre Kazuo Kuniyoshi
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 06/52695-9 - Alteracoes locais induzidas pela secrecao toxica de philodryas patagoniensis (girard, 1857) (serpentes: colubridae).
• Beneficiário: Priscila Hess Lopes
• Modalidade de apoio: Bolsas no Brasil - Mestrado