| Texto completo | |
| Autor(es): Mostrar menos - |
Elainy Patricia Lino Gasparotto
[1]
;
Raquel Tognon
[2]
;
Aline Fernanda Ferreira
[3]
;
Gislane Lelis Vilela Oliveira
[4]
;
Patrícia Vianna Bonini Palma
[5]
;
Maria Aparecida Zanichelli
[6]
;
Elizabeth Xisto Souto
[7]
;
Carlos Eduardo Engel Velano
[8]
;
Belinda Pinto Simões
[9]
;
Rita de Cassia Viu Carrara
[10]
;
Simone Kashima
[11]
;
Dimas Tadeu Covas
;
Fabíola Attie de Castro
[13]
;
Ana Maria de Souza
[14]
Número total de Autores: 14
|
| Afiliação do(s) autor(es): Mostrar menos - | [1] University of São Paulo. Faculty of Pharmaceutical Sciences of Ribeirão Preto. Department of Clinical Analyses, Toxicology and Food Sciences
[2] University of São Paulo. Faculty of Pharmaceutical Sciences of Ribeirão Preto. Department of Clinical Analyses, Toxicology and Food Sciences
[3] University of São Paulo. Faculty of Pharmaceutical Sciences of Ribeirão Preto. Department of Clinical Analyses, Toxicology and Food Sciences
[4] University of São Paulo. Faculty of Pharmaceutical Sciences of Ribeirão Preto. Department of Clinical Analyses, Toxicology and Food Sciences
[5] University of São Paulo. School of Medicine of Ribeirão Preto. Hemotherapy Center of Ribeirão Preto
[6] Brigadeiro Hospital
[7] Brigadeiro Hospital
[8] University of São Paulo. Faculty of Medicine of Ribeirão Preto. Department of Clinical Medicine
[9] University of São Paulo. Faculty of Medicine of Ribeirão Preto. Department of Clinical Medicine
[10] University of São Paulo. School of Medicine of Ribeirão Preto. Hemotherapy Center of Ribeirão Preto
[11] University of São Paulo. School of Medicine of Ribeirão Preto. Hemotherapy Center of Ribeirão Preto
[13] University of São Paulo. Faculty of Pharmaceutical Sciences of Ribeirão Preto. Department of Clinical Analyses, Toxicology and Food Sciences
[14] University of São Paulo. Faculty of Pharmaceutical Sciences of Ribeirão Preto. Department of Clinical Analyses, Toxicology and Food Sciences
Número total de Afiliações: 14
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Brazilian Journal of Pharmaceutical Sciences; v. 47, n. 4, p. 873-886, 2011-12-00. |
| Resumo | |
Apoptosis deregulation might have a role in the pathophysiology of polycythemia vera (PV). This study evaluated Bcl-2 molecule expression in CD34+ cells and leukocytes in 12 PV patients. Gene expression was investigated by real time PCR using SybrGreen Quantitect kit and protein expression was evaluated by western-blotting. JAK2 V617F mutation was detected according to Baxter et al (2005). CD34+ cells from PV patients presented higher levels of A1 and Mcl-1 expression (median: 22.6 and 5.2, respectively) in comparison with controls (0.9 and 0.5, p=0.004 and p=0.020); while Bcl-2 and Bcl-xL expression decreased in PV patients (0.18 and 1.19) compared with controls (1.39 and 2.01, p=0.006 and p=0.020). CD34+ cells in PV patients showed an elevated Bid expression (14.4) in comparison with healthy subjects (1.0; p=0.002). Patients' leukocytes showed an A1 augmentation (7.41, p=0.001) and a reduced expression of Bax (0.19; p=0.040) and Bad (0.2; p=0.030). There was no correlation between JAK2 V617F allele burden and molecular expression. PV patients showed alterations in Bcl-2 members' expression, which may interfere with control of apoptotic machinery and contribute to disease pathogenesis. (AU) | |
| Processo FAPESP: | 06/50094-8 - Expressão de gênese de proteínas pró e antiapoptóticas em doenças mieloproliferativas crônicas |
| Beneficiário: | Fabíola Attié de Castro |
| Modalidade de apoio: | Auxílio à Pesquisa - Jovens Pesquisadores |