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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Deregulated expression of A1, Bcl-2, Bcl-xL, and Mcl-1 antiapoptotic proteins and Bid, Bad, and Bax proapoptotic genes in polycythemia vera patients

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Author(s):
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Elainy Patricia Lino Gasparotto [1] ; Raquel Tognon [2] ; Aline Fernanda Ferreira [3] ; Gislane Lelis Vilela Oliveira [4] ; Patrícia Vianna Bonini Palma [5] ; Maria Aparecida Zanichelli [6] ; Elizabeth Xisto Souto [7] ; Carlos Eduardo Engel Velano [8] ; Belinda Pinto Simões [9] ; Rita de Cassia Viu Carrara [10] ; Simone Kashima [11] ; Dimas Tadeu Covas ; Fabíola Attie de Castro [13] ; Ana Maria de Souza [14]
Total Authors: 14
Affiliation:
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[1] University of São Paulo. Faculty of Pharmaceutical Sciences of Ribeirão Preto. Department of Clinical Analyses, Toxicology and Food Sciences
[2] University of São Paulo. Faculty of Pharmaceutical Sciences of Ribeirão Preto. Department of Clinical Analyses, Toxicology and Food Sciences
[3] University of São Paulo. Faculty of Pharmaceutical Sciences of Ribeirão Preto. Department of Clinical Analyses, Toxicology and Food Sciences
[4] University of São Paulo. Faculty of Pharmaceutical Sciences of Ribeirão Preto. Department of Clinical Analyses, Toxicology and Food Sciences
[5] University of São Paulo. School of Medicine of Ribeirão Preto. Hemotherapy Center of Ribeirão Preto
[6] Brigadeiro Hospital
[7] Brigadeiro Hospital
[8] University of São Paulo. Faculty of Medicine of Ribeirão Preto. Department of Clinical Medicine
[9] University of São Paulo. Faculty of Medicine of Ribeirão Preto. Department of Clinical Medicine
[10] University of São Paulo. School of Medicine of Ribeirão Preto. Hemotherapy Center of Ribeirão Preto
[11] University of São Paulo. School of Medicine of Ribeirão Preto. Hemotherapy Center of Ribeirão Preto
[13] University of São Paulo. Faculty of Pharmaceutical Sciences of Ribeirão Preto. Department of Clinical Analyses, Toxicology and Food Sciences
[14] University of São Paulo. Faculty of Pharmaceutical Sciences of Ribeirão Preto. Department of Clinical Analyses, Toxicology and Food Sciences
Total Affiliations: 14
Document type: Journal article
Source: Brazilian Journal of Pharmaceutical Sciences; v. 47, n. 4, p. 873-886, 2011-12-00.
Abstract

Apoptosis deregulation might have a role in the pathophysiology of polycythemia vera (PV). This study evaluated Bcl-2 molecule expression in CD34+ cells and leukocytes in 12 PV patients. Gene expression was investigated by real time PCR using SybrGreen Quantitect kit and protein expression was evaluated by western-blotting. JAK2 V617F mutation was detected according to Baxter et al (2005). CD34+ cells from PV patients presented higher levels of A1 and Mcl-1 expression (median: 22.6 and 5.2, respectively) in comparison with controls (0.9 and 0.5, p=0.004 and p=0.020); while Bcl-2 and Bcl-xL expression decreased in PV patients (0.18 and 1.19) compared with controls (1.39 and 2.01, p=0.006 and p=0.020). CD34+ cells in PV patients showed an elevated Bid expression (14.4) in comparison with healthy subjects (1.0; p=0.002). Patients' leukocytes showed an A1 augmentation (7.41, p=0.001) and a reduced expression of Bax (0.19; p=0.040) and Bad (0.2; p=0.030). There was no correlation between JAK2 V617F allele burden and molecular expression. PV patients showed alterations in Bcl-2 members' expression, which may interfere with control of apoptotic machinery and contribute to disease pathogenesis. (AU)