| Texto completo | |
| Autor(es): |
Martins-Pinheiro, Marinalva
;
Oliveira, Alice R.
;
Valencia, Alexy O.
;
Fernandez-Silva, Frank S.
;
Silva, Larissa G.
;
Lopes-Kulishev, Carina O.
;
Italiani, Valeria C. S.
;
Marques, Marilis V.
;
Menck, Carlos F.
;
Galhardo, Rodrigo S.
Número total de Autores: 10
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Gene; v. 626, p. 251-257, AUG 30 2017. |
| Citações Web of Science: | 1 |
| Resumo | |
Mutator strains were identified by screening random Tn5 insertion clones of Caulobacter crescentus. We identified clones with robust increases in mutation rates with Tn5 insertions in the mutY, mutS, mutL and uvrD genes, known to act in mutation-preventing pathways in Escherichia con. Analysis of mutations in the rpoB gene revealed that in both the parental strain and mismatch repair-deficient mutants, A:T -> G:C transitions predominate by a large margin over C:G -> T:A. We have also investigated the role of the error-prone polymerase encoded by imuC (dnaE2) in spontaneous mutagenesis, and found that a imuC mutant strain shows mutation rates and sequences comparable to the parental strain. Our study characterizes for the first time mutator strains in a member of the alphaproteobacteria group. In spite of the limitations of using a single marker, possible reasons for the observed mutational bias are discussed in the light of the repertoire of DNA repair genes in this bacterium. (AU) | |
| Processo FAPESP: | 14/15982-6 - Consequências de deficiências de reparo de lesões no genoma |
| Beneficiário: | Carlos Frederico Martins Menck |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 09/51387-7 - Regulação da mutagênese no modelo de Caulobacter crescentus e suas implicações para a evolução bacteriana |
| Beneficiário: | Rodrigo da Silva Galhardo |
| Modalidade de apoio: | Auxílio à Pesquisa - Jovens Pesquisadores |