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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Molecular characterization of Caulobacter crescentus mutator strains

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Author(s):
Martins-Pinheiro, Marinalva ; Oliveira, Alice R. ; Valencia, Alexy O. ; Fernandez-Silva, Frank S. ; Silva, Larissa G. ; Lopes-Kulishev, Carina O. ; Italiani, Valeria C. S. ; Marques, Marilis V. ; Menck, Carlos F. ; Galhardo, Rodrigo S.
Total Authors: 10
Document type: Journal article
Source: Gene; v. 626, p. 251-257, AUG 30 2017.
Web of Science Citations: 1
Abstract

Mutator strains were identified by screening random Tn5 insertion clones of Caulobacter crescentus. We identified clones with robust increases in mutation rates with Tn5 insertions in the mutY, mutS, mutL and uvrD genes, known to act in mutation-preventing pathways in Escherichia con. Analysis of mutations in the rpoB gene revealed that in both the parental strain and mismatch repair-deficient mutants, A:T -> G:C transitions predominate by a large margin over C:G -> T:A. We have also investigated the role of the error-prone polymerase encoded by imuC (dnaE2) in spontaneous mutagenesis, and found that a imuC mutant strain shows mutation rates and sequences comparable to the parental strain. Our study characterizes for the first time mutator strains in a member of the alphaproteobacteria group. In spite of the limitations of using a single marker, possible reasons for the observed mutational bias are discussed in the light of the repertoire of DNA repair genes in this bacterium. (AU)

FAPESP's process: 14/15982-6 - Consequences of repair deficiencies in damaged genome
Grantee:Carlos Frederico Martins Menck
Support type: Research Projects - Thematic Grants