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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Arginase expression modulates nitric oxide production in Leishmania (Leishmania) amazonensis

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Autor(es):
Acuna, Stephanie Maia [1] ; Aoki, Juliana Ide [1] ; Laranjeira-Silva, Maria Fernanda [1] ; Zampieri, Ricardo Andrade [1] ; Ribeiro Fernandes, Juliane Cristina [1] ; Muxel, Sandra Marcia [1] ; Floeter-Winter, Lucile Maria [1]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Inst Biociencias, Dept Fisiol, Sao Paulo - Brazil
Número total de Afiliações: 1
Tipo de documento: Artigo Científico
Fonte: PLoS One; v. 12, n. 11 NOV 14 2017.
Citações Web of Science: 10
Resumo

Background Arginase is an enzyme that converts L-arginine to urea and L-ornithine, an essential substrate for the polyamine pathway supporting Leishmania (Leishmania) amazonensis replication and its survival in the mammalian host. L-arginine is also the substrate of macrophage nitric oxide synthase 2 (NOS2) to produce nitric oxide (NO) that kills the parasite. This competition can define the fate of Leishmania infection. Methodology/Principal findings The transcriptomic profiling identified a family of oxidoreductases in L. (L.) amazonensis wild-type (La-WT) and L. (L.) amazonensis arginase knockout (La-arg(-)) promastigotes and axenic amastigotes. We highlighted the identification of an oxidoreductase that could act as nitric oxide synthase-like (NOS-like), due to the following evidences: conserved domain composition, the participation of NO production during the time course of promastigotes growth and during the axenic amastigotes differentiation, regulation dependence on arginase activity, as well as reduction of NO amount through the NOS activity inhibition. NO quantification was measured by DAF-FM labeling analysis in a flow cytometry. Conclusions/Significance We described an arginase-dependent NOS-like activity in L. (L.) amazonensis and its role in the parasite growth. The increased detection of NO production in the mid-stationary and late-stationary growth phases of La-WT promastigotes could suggest that this production is an important factor to metacyclogenesis triggering. On the other hand, La-arg(-) showed an earlier increase in NO production compared to La-WT, suggesting that NO production can be arginase-dependent. Interestingly, La-WT and La-arg(-) axenic amastigotes produced higher levels of NO than those observed in promastigotes. As a conclusion, our work suggested that NOS-like is expressed in Leishmania in the stationary growth phase promastigotes and amastigotes, and could be correlated to metacyclogenesis and amastigotes growth in a dependent way to the internal pool of L-arginine and arginase activity. (AU)

Processo FAPESP: 15/25942-4 - Estabelecimento de perfil de miRNA de macrófagos murinos incfetados por Leishmania (L.) amazonensis
Beneficiário:Stephanie Maia Acuna
Linha de fomento: Bolsas no Brasil - Iniciação Científica
Processo FAPESP: 14/50717-1 - Estudos bioquímicos, moleculares e funcionais da relação Leishmania-macrófago
Beneficiário:Lucile Maria Floeter-Winter
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 16/19815-2 - Papel dos microRNAs na resposta imune mediada por TLRs na infecção por Leishmania amazonensis.
Beneficiário:Sandra Marcia Muxel
Linha de fomento: Auxílio à Pesquisa - Regular