Bioprospection of Eugenia brasiliensis, a Brazilian native fruit, as a source of anti-inflammatory and antibiofilm compounds

Autor(es)

Lazarini, Josy Goldoni ^[1] ; Orlandi Sardi, Janaina de Cassia ^[1] ; Franchin, Marcelo ^[1] ; Nani, Bruno Dias ^[1] ; Freires, Irlan Almeida ^[2] ; Infante, Juliana ^[3] ; Rizzato Paschoal, Jonas Augusto ^[4] ; de Alencar, Severino Matias ^[3] ; Rosalen, Pedro Luiz ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Physiol Sci, Ave Limeira 901, BR-13414903 Piracicaba, SP - Brazil

^[2] Univ Florida, Coll Dent, Dept Oral Biol, 1395 Ctr Dr, Gainesville, FL 32610 - USA

^[3] Univ Sao Paulo, Luiz de Queiroz Coll Agr, Dept Agrifood Ind Food & Nutr, Ave Padua Dias 11, BR-13418900 Piracicaba, SP - Brazil

^[4] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Dept Phys & Chem, Ave Cafe, BR-14040903 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BIOMEDICINE & PHARMACOTHERAPY; v. 102, p. 132-139, JUN 2018.
• Citações Web of Science: 11

Resumo

The anti-inflammatory and antibiofilm activities as well as toxicity and chemical profile of Eugenia brasiliensis pulp extract (EBE), were evaluated. EBE chemical profile and phenolic content were determined by LC-MS/MS. EBE was tested for its in vitro and in vivo anti-inflammatory activity, including TNF-alpha release, NF-kB activation, neutrophil migration and paw edema. The MIC/MBC and antibiofilm activities were tested against methicillin sensitive and resistant Staphylococcus aureus, Escherichia coli, Pseudomona aeruginosa, Streptococcus mutans, and Lactobacillus acidophilus. EBE acute toxicity was evaluated in Galleria mellonella and RAW 264.7 macrophage. EBE total phenolic content was 389.88 +/- 3.48 mg GAE/g with identified polyphenols. EBE decreased TNF-alpha release in vivo and in vitro, NF-kB activation, neutrophil influx into peritoneal cavity, and it showed maximal inhibition of paw edema after 2 h. MIC of EBE ranged from 62.5-500 mu g/mL while MBC values were > 500 mu g/mL, with a decrease in L. acidophilus biofilm formation. EBE showed negligible toxicity in larvae and macrophage cells. Our findings open new perspectives concerning EBE application as source of anti-inflammatory and antibiofilm molecules as a functional food, pharmaceutical lead or agribusiness commodity. (AU)

• Processo FAPESP: 13/26251-0 - Prospecção de atividade anti-inflamatória em espécies frutíferas nativas do Brasil
• Beneficiário: Josy Goldoni Lazarini
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 13/13190-2 - Composição fenólica e potencial biológico, in vitro e in vivo, de frutíferas nativas do Brasil
• Beneficiário: Severino Matias de Alencar
• Modalidade de apoio: Auxílio à Pesquisa - Regular