Replication Protein A-1 Has a Preference for the Telomeric G-rich Sequence in Trypanosoma cruzi

Autor(es)

Pavani, Raphael Souza ^{[1, 2]} ; Vitarelli, Marcela O. ^{[1, 2]} ; Fernandes, Carlos A. H. ^[3] ; Mattioli, Fabio F. ^[3] ; Morone, Mariana ^{[4, 2]} ; Menezes, Milene C. ^{[4, 2]} ; Fontes, Marcos R. M. ^[3] ; Cano, Maria Isabel N. ^[5] ; Elias, Maria Carolina ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Inst Butantan, Lab Especial Ciclo Celular, BR-05503900 Sao Paulo, SP - Brazil

^[2] Inst Butantan, Ctr Toxins Immune Response & Cell Signaling CeTIC, BR-05503900 Sao Paulo, SP - Brazil

^[3] Sao Paulo State Univ UNESP, Biosci Inst, Biophys & Phys Dept, BR-18618970 Botucatu, SP - Brazil

^[4] Inst Butantan, Lab Especial Toxinol Aplicada, BR-05503900 Sao Paulo, SP - Brazil

^[5] Sao Paulo State Univ UNESP, Biosci Inst, Genet Dept, BR-18618970 Botucatu, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Eukaryotic Microbiology; v. 65, n. 3, p. 345-356, MAY-JUN 2018.
• Citações Web of Science: 2

Resumo

Replication protein A (RPA), the major eukaryotic single-stranded binding protein, is a heterotrimeric complex formed by RPA-1, RPA-2, and RPA-3. RPA is a fundamental player in replication, repair, recombination, and checkpoint signaling. In addition, increasing evidences have been adding functions to RPA in telomere maintenance, such as interaction with telomerase to facilitate its activity and also involvement in telomere capping in some conditions. Trypanosoma cruzi, the etiological agent of Chagas disease is a protozoa parasite that appears early in the evolution of eukaryotes. Recently, we have showed that T. cruzi RPA presents canonical functions being involved with DNA replication and DNA damage response. Here, we found by FISH/IF assays that T. cruzi RPA localizes at telomeres even outside replication (S) phase. In vitro analysis showed that one telomeric repeat is sufficient to bind RPA-1. Telomeric DNA induces different secondary structural modifications on RPA-1 in comparison with other types of DNA. In addition, RPA-1 presents a higher affinity for telomeric sequence compared to randomic sequence, suggesting that RPA may play specific roles in T. cruzi telomeric region. (AU)

• Processo FAPESP: 13/07467-1 - CeTICS - Centro de Toxinas, Imuno-Resposta e Sinalização Celular
• Beneficiário: Hugo Aguirre Armelin
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 15/10580-0 - Caracterização de checkpoint intra-S em células de Trypanosoma
• Beneficiário: Maria Carolina Quartim Barbosa Elias Sabbaga
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/02978-0 - Análise funcional do complexo RPA em Trypanosoma cruzi e seu envolvimento com DNA telomérico
• Beneficiário: Raphael Souza Pavani
• Modalidade de apoio: Bolsas no Brasil - Doutorado