Replication Protein A-1 Has a Preference for the Telomeric G-rich Sequence in Trypanosoma cruzi

Author(s)

Pavani, Raphael Souza ^{[1, 2]} ; Vitarelli, Marcela O. ^{[1, 2]} ; Fernandes, Carlos A. H. ^[3] ; Mattioli, Fabio F. ^[3] ; Morone, Mariana ^{[4, 2]} ; Menezes, Milene C. ^{[4, 2]} ; Fontes, Marcos R. M. ^[3] ; Cano, Maria Isabel N. ^[5] ; Elias, Maria Carolina ^{[1, 2]}

Affiliation

^[1] Inst Butantan, Lab Especial Ciclo Celular, BR-05503900 Sao Paulo, SP - Brazil

^[2] Inst Butantan, Ctr Toxins Immune Response & Cell Signaling CeTIC, BR-05503900 Sao Paulo, SP - Brazil

^[3] Sao Paulo State Univ UNESP, Biosci Inst, Biophys & Phys Dept, BR-18618970 Botucatu, SP - Brazil

^[4] Inst Butantan, Lab Especial Toxinol Aplicada, BR-05503900 Sao Paulo, SP - Brazil

^[5] Sao Paulo State Univ UNESP, Biosci Inst, Genet Dept, BR-18618970 Botucatu, SP - Brazil

• Document type: Journal article
• Source: Journal of Eukaryotic Microbiology; v. 65, n. 3, p. 345-356, MAY-JUN 2018.
• Web of Science Citations: 2

Abstract

Replication protein A (RPA), the major eukaryotic single-stranded binding protein, is a heterotrimeric complex formed by RPA-1, RPA-2, and RPA-3. RPA is a fundamental player in replication, repair, recombination, and checkpoint signaling. In addition, increasing evidences have been adding functions to RPA in telomere maintenance, such as interaction with telomerase to facilitate its activity and also involvement in telomere capping in some conditions. Trypanosoma cruzi, the etiological agent of Chagas disease is a protozoa parasite that appears early in the evolution of eukaryotes. Recently, we have showed that T. cruzi RPA presents canonical functions being involved with DNA replication and DNA damage response. Here, we found by FISH/IF assays that T. cruzi RPA localizes at telomeres even outside replication (S) phase. In vitro analysis showed that one telomeric repeat is sufficient to bind RPA-1. Telomeric DNA induces different secondary structural modifications on RPA-1 in comparison with other types of DNA. In addition, RPA-1 presents a higher affinity for telomeric sequence compared to randomic sequence, suggesting that RPA may play specific roles in T. cruzi telomeric region. (AU)

• FAPESP's process: 13/07467-1 - CeTICS - Center of Toxins, Immune-Response and Cell Signaling
• Grantee: Hugo Aguirre Armelin
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
• FAPESP's process: 15/10580-0 - Characterization of intra-S checkpoint in Trypanosoma cells
• Grantee: Maria Carolina Quartim Barbosa Elias Sabbaga
• Support Opportunities: Regular Research Grants
• FAPESP's process: 14/02978-0 - Functional analysis of RPA complex in Trypanosoma cruzi and its involvement with telomeric DNA
• Grantee: Raphael Souza Pavani
• Support Opportunities: Scholarships in Brazil - Doctorate