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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Amastigote Synapse: The Tricks of Trypanosoma cruzi Extracellular Amastigotes

Texto completo
Autor(es):
Bonfim-Melo, Alexis [1] ; Ferreira, Eden R. [1] ; Florentino, Pilar T. V. [2] ; Mortara, Renato A. [1]
Número total de Autores: 4
Afiliação do(s) autor(es):
[1] Univ Fed Sao Paulo, Dept Microbiol Imunol & Parasitol, Escola Paulista Med, Sao Paulo - Brazil
[2] Univ Sao Paulo, Biomed Sci Inst 2, DNA Repair Lab, Sao Paulo - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo de Revisão
Fonte: FRONTIERS IN MICROBIOLOGY; v. 9, JUN 27 2018.
Citações Web of Science: 3
Resumo

To complete its life cycle within the mammalian host, Trypanosoma cruzi, the agent of Chagas' disease, must enter cells. Trypomastigotes originating from the insect vector (metacyclic) or from infected cells (bloodstream/tissue culture-derived) are the classical infective forms of the parasite and enter mammalian cells in an actin-independent manner. By contrast, amastigotes originating from the premature rupture of infected cells or transformed from swimming trypomastigotes (designated extracellular amastigotes, EAs) require functional intact microfilaments to invade non-phagocytic host cells. Earlier work disclosed the key features of EA-HeLa cell interplay: actin-rich protrusions called `cups' are formed at EA invasion sites on the host cell membrane that are also enriched in actin-binding proteins, integrins and extracellular matrix elements. In the past decades we described the participation of membrane components and secreted factors from EAs as well as the actin-regulating proteins of host cells involved in what we propose to be a phagocytic-like mechanism of parasite uptake. Thus, regarding this new perspective herein we present previously described EA-induced `cups' as parasitic synapse since they can play a role beyond its architecture function. In this review, we focus on recent findings that shed light on the intricate interaction between extracellular amastigotes and non-phagocytic HeLa cells. (AU)

Processo FAPESP: 11/51475-3 - Biologia molecular e celular do parasitismo por Trypanosoma cruzi
Beneficiário:José Franco da Silveira Filho
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 11/03357-1 - Caracterização do perfil de expressão de epítopos e infectividade de amastigotas extracelulares de diferentes isolados de Trypanosoma cruzi
Beneficiário:Pilar Sampaio Tavares Veras
Linha de fomento: Bolsas no Brasil - Mestrado
Processo FAPESP: 12/21335-8 - As vias de Cdc42/N-WASP e Rac1/WAVE2 na dinâmica de actina durante a invasão celular pelos amastigotas extracelulares de Trypanosoma cruzi
Beneficiário:Alexis de Sá Ribeiro do Bonfim de Melo
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 12/25282-6 - Participação das proteínas ezrina, radixina e moesina (ERM proteins) na invasão celular por amastigotas extracelulares de Trypanosoma cruzi.
Beneficiário:Éden Ramalho de Araujo Ferreira
Linha de fomento: Bolsas no Brasil - Doutorado