Potential of the association of dodecyl gallate with nanostructured lipid system as a treatment for paracoccidioidomycosis: In vitro and in vivo efficacy and toxicity

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Singulani, Junya de Lacorte ^[1] ; Scorzoni, Liliana ^[1] ; Strohmayer Lourencetti, Natalia Manuela ^[1] ; Oliveira, Luana Rossi ^[1] ; Concoaro, Rosana Silva ^[1] ; da Silva, Patricia Bento ^[1] ; Nazare, Ana Carolina ^[2] ; Polaquini, Carlos Roberto ^[2] ; Victorelli, Francesca Damiani ^[1] ; Chorilli, Marlus ^[1] ; Regasini, Luis Octavio ^[2] ; Fusco Almeida, Ana Marisa ^[1] ; Soares Mendes Giannini, Maria Jose ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Sch Pharmaceut Sci, Rodovia Araraquara Jau Km 1, BR-14800903 Araraquara, SP - Brazil

^[2] Sao Paulo State Univ UNESP, Inst Biosci Letters & Exact Sci, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: International Journal of Pharmaceutics; v. 547, n. 1-2, p. 630-636, AUG 25 2018.
• Citações Web of Science: 1

Resumo

Paracoccidioidomycosis (PCM) is a systemic mycosis endemic in Latin America, caused by Paracoccidioides spp. A limited number of antifungal agents are available and the search for new compounds has increased. Additionally, nanostructured lipid system (NLS) has emmerged as an interesting strategy to carrier compounds for the treatment of mycosis. In this work, the antifungal efficacy and toxicity of dodecyl gallate (DOD) associated with a NLS was evaluated through in vitro and in vivo tests. DOD showed good in vitro antifungal activity and low toxicity in lung fibroblasts and zebrafish embryos, but no antifungal efficacy in infected mice, which may have been a result of low bioavailability. On the other hand, the association of DOD + NLS was beneficial and resulted in lower toxicity in lung fibroblasts and zebrafish embryos. In addition, NLS + DOD promoted a significant reduction in the fungal burden of mice lungs and could be a potential therapeutic option against PCM. (AU)

• Processo FAPESP: 13/10917-9 - Modelos animais alternativos: Virulência de diferentes espécies filogenéticas de Paracoccidioides e efeito das proteínas 43kDa e 30kDa e de seus anticorpos
• Beneficiário: Liliana Scorzoni
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 14/10446-9 - Pesquisa de novas terapias e de biomarcadores na paracoccidioidomicose: estudo da atividade antifúngica de galatos de alquila em modelos alternativos e camundongos e identificação de microRNAs circulantes
• Beneficiário: Junya de Lacorte Singulani
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 15/03700-9 - Animais alternativos aplicados à interação Paracoccidioides-hospedeiro para o desenvolvimento de estudos de virulência, eficácia e toxicidade de compostos antifúngicos e tratamentos preventivos
• Beneficiário: Maria José Soares Mendes Giannini
• Modalidade de apoio: Auxílio à Pesquisa - Regular