Potential of the association of dodecyl gallate with nanostructured lipid system as a treatment for paracoccidioidomycosis: In vitro and in vivo efficacy and toxicity

Author(s):Show less -

Singulani, Junya de Lacorte ^[1] ; Scorzoni, Liliana ^[1] ; Strohmayer Lourencetti, Natalia Manuela ^[1] ; Oliveira, Luana Rossi ^[1] ; Concoaro, Rosana Silva ^[1] ; da Silva, Patricia Bento ^[1] ; Nazare, Ana Carolina ^[2] ; Polaquini, Carlos Roberto ^[2] ; Victorelli, Francesca Damiani ^[1] ; Chorilli, Marlus ^[1] ; Regasini, Luis Octavio ^[2] ; Fusco Almeida, Ana Marisa ^[1] ; Soares Mendes Giannini, Maria Jose ^[1]

Affiliation

^[1] Sao Paulo State Univ UNESP, Sch Pharmaceut Sci, Rodovia Araraquara Jau Km 1, BR-14800903 Araraquara, SP - Brazil

^[2] Sao Paulo State Univ UNESP, Inst Biosci Letters & Exact Sci, Sao Paulo - Brazil

• Document type: Journal article
• Source: International Journal of Pharmaceutics; v. 547, n. 1-2, p. 630-636, AUG 25 2018.
• Web of Science Citations: 1

Abstract

Paracoccidioidomycosis (PCM) is a systemic mycosis endemic in Latin America, caused by Paracoccidioides spp. A limited number of antifungal agents are available and the search for new compounds has increased. Additionally, nanostructured lipid system (NLS) has emmerged as an interesting strategy to carrier compounds for the treatment of mycosis. In this work, the antifungal efficacy and toxicity of dodecyl gallate (DOD) associated with a NLS was evaluated through in vitro and in vivo tests. DOD showed good in vitro antifungal activity and low toxicity in lung fibroblasts and zebrafish embryos, but no antifungal efficacy in infected mice, which may have been a result of low bioavailability. On the other hand, the association of DOD + NLS was beneficial and resulted in lower toxicity in lung fibroblasts and zebrafish embryos. In addition, NLS + DOD promoted a significant reduction in the fungal burden of mice lungs and could be a potential therapeutic option against PCM. (AU)

• FAPESP's process: 15/03700-9 - Alternative animal as a model to study Paracoccidioides- host interaction: virulence, efficacy and toxicology of antifungal compounds and new preventive treatments
• Grantee: Maria José Soares Mendes Giannini
• Support type: Regular Research Grants
• FAPESP's process: 14/10446-9 - Evaluation of new therapies and biomarkers in paracoccidioidomycosis: antifungal activity of alkyl gallates in alternative models and mice and identification of circulating microRNAs
• Grantee: Junya de Lacorte Singulani
• Support type: Scholarships in Brazil - Doctorate
• FAPESP's process: 13/10917-9 - Alternative animal models: Virulence of different phylogenetic species of Paracoccidioides and effect of the 30kDa 43kDa protein and their antibodies
• Grantee: Liliana Scorzoni
• Support type: Scholarships in Brazil - Post-Doctorate