Alternative animal models: Virulence of different phylogenetic species of Paracoccidioides and effect of the 30kDa 43kDa protein and their antibodies

• Grant number: 13/10917-9
• Support type: Scholarships in Brazil - Post-Doctorate
• Effective date (Start): September 01, 2013
• Effective date (End): December 31, 2016
• Field of knowledge: Health Sciences - Pharmacy
• Principal Investigator: Maria José Soares Mendes Giannini
• Grantee: Liliana Scorzoni
• Home Institution: Faculdade de Ciências Farmacêuticas (FCFAR). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
• Associated scholarship(s): 15/14459-0 - Use of holograph optical trap: image system to study the adhesin function of Drechmeria coniospora 14-3-3 protein in Caenorhabditis elegans, BE.EP.PD

Abstract

The use of alternative animal models is increasingly spreading due to the ethical difficulties for in vivo test in mammals. Galleria mellonella (Lepidoptera: Pyralidae) has been used to study the virulence of microorganisms. This organism has six types of phagocytic cells which play an important role in the defense system, the cells density in the haemolymph is not constant and can be used as a virulence parameter and the possibility of incubating larvae from 25 to 37 ° C makes the model even more versatile. Caenorhabditis elegans is sensitive to bacterial infection and fungal pathogens, is easy to maintain and reproduce in the laboratory, the presence of the immune system to recognize and eliminate pathogens has high genetic homology with vertebrates; besides, C. elegans has its genome completely known. The complex Paracoccidioides spp are dimorphic fungus and endemic throughout Latin America. Phylogenetic studies indicate the existence of three different phylogenetic species of Paracoccidioides which are able to infect humans: S1, S2 and PS3. Infection is by inhalation, and then the fungus chance for yeast morphology. The first organ to be affected is the lung and then the infection can spread to other tissues causing systemic mycosis. The fungus adhesion to host cells is a key factor for established the infection. Thus, different molecules have been described with adhesion function in the course of infection like Gp-43, enolase, 30kDa, GAPDH, malate synthase and triofosfatase isomerase. In this context, glycoprotein of 43 kDa and 30 kDa protein (14-3-3 family) has been widely studied. In this project, we intend to evaluate the in vivo virulence of different phylogenetic species belonging to the Paracoccidioides complex in animal alternative models: G. mellonella and C. elegans. Also evaluate the influence of treatments of glycoprotein of 43 kDa and 30 kDa protein and its antibodies in the survival of these animals and finally to evaluate the gene expression of Paracoccidioides adhesins during infection

Scientific publications

(16)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SCORZONI, LILIANA; ALVES DE PAULA E SILVA, ANA CAROLINA; DE OLIVEIRA, HAROLDO CESAR; DOS SANTOS, CLAUDIA TAVARES; SINGULANI, JUNYA DE LACORTE; ASSATO, PATRICIA AKEMI; MARCOS, CAROLINE MARIA; OLIVEIRA, LARIANE TEODORO; FREGONEZI, NATHALIA FERREIRA; PEREIRA ROSSI, DIEGO CONRADO; ROQUE DA SILVA, LEANDRO BUFFONI; TABORDA, CARLOS PELLESCHI; FUSCO-ALMEIDA, ANA MARISA; SOARES MENDES-GIANNINI, MARIA JOSE. In Vitro and In Vivo Effect of Peptides Derived from 14-3-3 Paracoccidioides spp. Protein. JOURNAL OF FUNGI, v. 7, n. 1 JAN 2021. Web of Science Citations: 0.

MARCOS, CAROLINE MARIA; TAMER, GABRIELLE; DE OLIVEIRA, HAROLDO CESAR; ASSATO, PATRICIA AKEMI; SCORZONI, LILIANA; SANTOS, CLAUDIA TAVARES; SINGULANI, JUNYA DE LACORTE; DA SILVA, JULHIANY DE FATIMA; DE ALMEIDA, RODRIGO; ALVES DE PAULA E SILVA, ANA CAROLINA; MORAES DA SILVA, ROSANGELA APARECIDA; DE ANDRADE, CLEVERTON ROBERTO; TAMAYO, DIANA PATRICIA; LOPEZ, ANGELA MARIA; BARBOSA, NATALIA MOREIRA; ZANELLI, CLESLEI FERNANDO; HERNANDEZ-RUIZ, ORVILLE; MCEWEN, JUAN G.; SOARES MENDES-GIANNINI, MARIA JOSE; FUSCO-ALMEIDA, ANA MARISA. Down-regulation of TUFM impairs host cell interaction and virulence by Paracoccidioides brasiliensis. SCIENTIFIC REPORTS, v. 9, NOV 20 2019. Web of Science Citations: 0.

ALVES DE PAULA E SILVA, ANA CAROLINA; DE OLIVEIRA, HAROLDO CESAR; SCORZONI, LILIANA; MARCOS, CAROLINE MARIA; DOS SANTOS, CLAUDIA TAVARES; FUSCO-ALMEIDA, ANA MARISA; GUERTA SALINA, ANA CAROLINA; MEDEIROS, ALEXANDRA IVO; ALMEIDA, FAUSTO; LI, SHEENA CLAIRE; BOONE, CHARLES; MENDES-GIANNINI, MARIA J. S. Decyl Gallate as a Possible Inhibitor of N-Glycosylation Process in Paracoccidioides lutzii. Antimicrobial Agents and Chemotherapy, v. 63, n. 11 NOV 2019. Web of Science Citations: 0.

SINGULANI, JUNYA DE LACORTE; SCORZONI, LILIANA; STROHMAYER LOURENCETTI, NATALIA MANUELA; OLIVEIRA, LUANA ROSSI; CONCOARO, ROSANA SILVA; DA SILVA, PATRICIA BENTO; NAZARE, ANA CAROLINA; POLAQUINI, CARLOS ROBERTO; VICTORELLI, FRANCESCA DAMIANI; CHORILLI, MARLUS; REGASINI, LUIS OCTAVIO; FUSCO ALMEIDA, ANA MARISA; SOARES MENDES GIANNINI, MARIA JOSE. Potential of the association of dodecyl gallate with nanostructured lipid system as a treatment for paracoccidioidomycosis: In vitro and in vivo efficacy and toxicity. International Journal of Pharmaceutics, v. 547, n. 1-2, p. 630-636, AUG 25 2018. Web of Science Citations: 1.

SCORZONI, LILIANA; ALVES DE PAULA E SILVA, ANA CAROLINA; DE OLIVEIRA, HAROLDO CESAR; MARCOS, CAROLINE MARIA; SINGULANI, JUNYA DE LACORTE; FUSCO-ALMEIDA, ANA MARISA; SOARES MENDES-GIANNINI, MARIA JOSE. Can passage in Galleria mellonella activate virulence factors of Paracoccidioides brasiliensis as in the murine model?. Medical Mycology, v. 56, n. 3, p. 374-377, APR 2018. Web of Science Citations: 2.

SCORZONI, LILIANA; PILAR DE LUCAS, MARIA; SINGULANI, JUNYA DE LACORTE; DE OLIVEIRA, HAROLDO CESAR; ASSATO, PATRICIA AKEMI; FUSCO-ALMEIDA, ANA MARISA; SOARES MENDES-GIANNINI, MARIA JOSE. Evaluation of &ITCaenorhabditis elegans&IT as a host model for &ITParacoccidioides brasiliensis &ITand &ITParacoccidioides lutzii&IT. PATHOGENS AND DISEASE, v. 76, n. 1 FEB 2018. Web of Science Citations: 1.

SINGULANI, JUNYA DE LACORTE; SCORZONI, LILIANA; GOMES, PAULO CESAR; NAZARE, ANA CAROLINA; POLAQUINI, CARLOS ROBERTO; REGASINI, LUIS OCTAVIO; FUSCO-ALMEIDA, ANA MARISA; SOARES MENDES-GIANNINI, MARIA JOSE. Activity of gallic acid and its ester derivatives in Caenorhabditis elegans and zebrafish (Danio rerio) models. Future Medicinal Chemistry, v. 9, n. 16, p. 1863-1872, OCT 2017. Web of Science Citations: 6.

COSTA-ORLANDI, CAROLINE B.; SARDI, JANAINA C. O.; PITANGUI, NAYLA S.; DE OLIVEIRA, HAROLDO C.; SCORZONI, LILIANA; GALEANE, MARIANA C.; MEDINA-ALARCON, KAILA P.; MELO, WANESSA C. M. A.; MARCELINO, MONICA Y.; BRAZ, JAQUELINE D.; FUSCO-ALMEIDA, ANA MARISA; MENDES-GIANNINI, MARIA JOSE S. Fungal Biofilms and Polymicrobial Diseases. JOURNAL OF FUNGI, v. 3, n. 2 JUN 2017. Web of Science Citations: 18.

SCORZONI, LILIANA; DE PAULA E SILVA, ANA C. A.; MARCOS, CAROLINE M.; ASSATO, PATRICIA A.; DE MELO, WANESSA C. M. A.; DE OLIVEIRA, HAROLDO C.; COSTA-ORLANDI, CAROLINE B.; MENDES-GIANNINI, MARIA J. S.; FUSCO-ALMEIDA, ANA M. Antifungal Therapy: New Advances in the Understanding and Treatment of Mycosis. FRONTIERS IN MICROBIOLOGY, v. 8, JAN 23 2017. Web of Science Citations: 66.

DE OLIVEIRA, HAROLDO C.; MICHALOSKI, JUSSARA S.; DA SILVA, JULHIANY F.; SCORZONI, LILIANA; DE PAULA E SILVA, ANA C. A.; MARCOS, CAROLINE M.; ASSATO, PATRICIA A.; YAMAZAKI, DANIELLE S.; FUSCO-ALMEIDA, ANA M.; GIORDANO, RICARDO J.; MENDES-GIANNINI, MARIA J. S. Peptides Derived from a Phage Display Library Inhibit Adhesion and Protect the Host against Infection by Paracoccidioides brasiliensis and Paracoccidioides lutzii. FRONTIERS IN PHARMACOLOGY, v. 7, DEC 23 2016. Web of Science Citations: 6.

MARCOS, CAROLINE M.; DE OLIVEIRA, HAROLDO C.; ANTUNES DE MELO, WANESSA DE CASSIA M.; DA SILVA, JULHIANY DE FATIMA; ASSATO, PATRICIA A.; SCORZONI, LILIANA; ROSSI, SUELEN A.; DE PAULA E SILVA, ANA C. A.; MENDES-GIANNINI, MARIA J. S.; FUSCO-ALMEIDA, ANA M. Anti-Immune Strategies of Pathogenic Fungi. FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, v. 6, NOV 15 2016. Web of Science Citations: 15.

SANGALLI-LEITE, FERNANDA; SCORZONI, LILIANA; ALVES DE PAULA E SILVA, ANA CAROLINA; DA SILVA, JULHIANY DE FATIMA; DE OLIVEIRA, HAROLDO CESAR; SINGULANI, JUNYA DE LACORTE; GULLO, FERNANDA PATRICIA; DA SILVA, ROSANGELA MORAES; REGASINI, LUIS OCTAVIO; SIQUEIRA DA SILVA, DULCE HELENA; BOLZANI, VANDERLAN DA SILVA; FUSCO-ALMEIDA, ANA MARISA; SOARES MENDES-GIANNINI, MARIA JOSE. Synergistic effect of pedalitin and amphotericin B against Cryptococcus neoformans by in vitro and in vivo evaluation. INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS, v. 48, n. 5, p. 504-511, NOV 2016. Web of Science Citations: 9.

SINGULANI, JUNYA DE LACORTE; SCORZONI, LILIANA; ALVES DE PAULA E SILVA, ANA CAROLINA; FUSCO-ALMEIDA, ANA MARISA; SOARES MENDES-GIANNINI, MARIA JOSE. Evaluation of the efficacy of antifungal drugs against Paracoccidioides brasiliensis and Paracoccidioides lutzii in a Galleria mellonella model. INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS, v. 48, n. 3, p. 292-297, SEP 2016. Web of Science Citations: 11.

SCORZONI, LILIANA; SANGALLI-LEITE, FERNANDA; SINGULANI, JUNYA DE LACORTE; ALVES DE PAULA E SILVA, ANA CAROLINA; COSTA-ORLANDI, CAROLINE BARCELOS; FUSCO-ALMEIDA, ANA MARISA; MENDES-GIANNINI, MARIA JOSE SOARES. Searching new antifungals: The use of in vitro and in vivo methods for evaluation of natural compounds. Journal of Microbiological Methods, v. 123, p. 68-78, APR 2016. Web of Science Citations: 16.

SCORZONI, LILIANA; ALVES DE PAULA E SILVA, ANA CAROLINA; SINGULANI, JUNYA DE LACORTE; LEITE, FERNANDA SANGALLI; DE OLIVEIRA, HAROLDO CESAR; MORAES DA SILVA, ROSANGELA APARECIDA; FUSCO-ALMEIDA, ANA MARISA; SOARES MENDES-GIANNINI, MARIA JOSE. Comparison of virulence between Paracoccidioides brasiliensis and Paracoccidioides lutzii using Galleria mellonella as a host model. VIRULENCE, v. 6, n. 8, p. 766-776, NOV 17 2015. Web of Science Citations: 13.

TREVIJANO-CONTADOR, NURIA; HERRERO-FERNANDEZ, INES; GARCIA-BARBAZAN, IRENE; SCORZONI, LILIANA; RUEDA, CRISTINA; ANDREIA ROSSI, SUELEN; GARCIA-RODAS, ROCIO; ZARAGOZA, OSCAR. Cryptococcus neoformans induces antimicrobial responses and behaves as a facultative intracellular pathogen in the non mammalian model Galleria mellonella. VIRULENCE, v. 6, n. 1, p. 66-74, JAN 2015. Web of Science Citations: 18.