Ouabain increases neuronal branching in hippocampus and improves spatial memory

Autor(es)

Orellana, Ana Maria ^[1] ; Leite, Jacqueline Alves ^[1] ; Kinoshita, Paula Fernanda ^[1] ; Vasconcelos, Andrea Rodrigues ^[1] ; Andreotta, Diana Zukas ^[1] ; Lima, Larissa de Sa ^[1] ; Xavier, Gilberto Fernando ^[2] ; Kawamoto, Elisa Mitiko ^[1] ; Scavone, Cristoforo ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biomed Sci 1, Dept Pharmacol, ICB I, Room 338, Av Prof Lineu Prestes, 1524, BR-05508900 Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Dept Physiol, Inst Biosci, Rua Matao, Travessa 14, 101, BR-05508090 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Neuropharmacology; v. 140, p. 260-274, SEP 15 2018.
• Citações Web of Science: 0

Resumo

Previous research shows Ouabain (OUA) to bind Na, K-ATPase, thereby triggering a number of signaling pathways, including the transcription factors NF kappa B and CREB. These transcription factors play a key role in the regulation of BDNF and WNT-beta-catenin signaling cascades, which are involved in neuroprotection and memory regulation. This study investigated the effects of OUA (10 nM) in the modulation of the principal signaling pathways involved in morphological plasticity and memory formation in the hippocampus of adult rats. The results show intrahippocampal injection of OUA 10 nM to activate the Wnt/beta-Catenin signaling pathway and to increase CREB/BDNF and NF kappa B levels. These effects contribute to important changes in the cellular micro environment, resulting in enhanced levels of dendritic branching in hippocampal neurons, in association with an improvement in spatial reference memory and the inhibition of long-term memory extinction. (AU)

• Processo FAPESP: 16/07427-8 - Envelhecimento e neuroproteção: ações da proteína Klotho no metabolismo energético, sinalização da Na,K-ATPase e respostas adaptativas no sistema nervoso central
• Beneficiário: Cristoforo Scavone
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 11/22844-0 - Estudo do mecanismo molecular de sinalização da ativação da via WNT/BETA-CATENINA pela Ouabaína em hipocampo de ratos.
• Beneficiário: Ana Maria Marques Orellana
• Modalidade de apoio: Bolsas no Brasil - Doutorado