Ouabain increases neuronal branching in hippocampus and improves spatial memory

Author(s)

Orellana, Ana Maria ^[1] ; Leite, Jacqueline Alves ^[1] ; Kinoshita, Paula Fernanda ^[1] ; Vasconcelos, Andrea Rodrigues ^[1] ; Andreotta, Diana Zukas ^[1] ; Lima, Larissa de Sa ^[1] ; Xavier, Gilberto Fernando ^[2] ; Kawamoto, Elisa Mitiko ^[1] ; Scavone, Cristoforo ^[1]

Affiliation

^[1] Univ Sao Paulo, Inst Biomed Sci 1, Dept Pharmacol, ICB I, Room 338, Av Prof Lineu Prestes, 1524, BR-05508900 Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Dept Physiol, Inst Biosci, Rua Matao, Travessa 14, 101, BR-05508090 Sao Paulo - Brazil

• Document type: Journal article
• Source: Neuropharmacology; v. 140, p. 260-274, SEP 15 2018.
• Web of Science Citations: 0

Abstract

Previous research shows Ouabain (OUA) to bind Na, K-ATPase, thereby triggering a number of signaling pathways, including the transcription factors NF kappa B and CREB. These transcription factors play a key role in the regulation of BDNF and WNT-beta-catenin signaling cascades, which are involved in neuroprotection and memory regulation. This study investigated the effects of OUA (10 nM) in the modulation of the principal signaling pathways involved in morphological plasticity and memory formation in the hippocampus of adult rats. The results show intrahippocampal injection of OUA 10 nM to activate the Wnt/beta-Catenin signaling pathway and to increase CREB/BDNF and NF kappa B levels. These effects contribute to important changes in the cellular micro environment, resulting in enhanced levels of dendritic branching in hippocampal neurons, in association with an improvement in spatial reference memory and the inhibition of long-term memory extinction. (AU)

• FAPESP's process: 16/07427-8 - Aging and neuroprotection: effects of Klotho protein in energetic metabolism, Na,K-ATPase signaling and adaptative response in central nervous system
• Grantee: Cristoforo Scavone
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 11/22844-0 - Study of the molecular mechanism activation of Wnt/Beta Catenin Signaling pathway by Ouabain in rat hippocampus.
• Grantee: Ana Maria Marques Orellana
• Support Opportunities: Scholarships in Brazil - Doctorate