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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Exercise and beta-alanine supplementation on carnosine-acrolein adduct in skeletal muscle

Texto completo
Autor(es):
Carvalho, Victor H. [1] ; Oliveira, Ana H. S. [1] ; de Oliveira, Luana F. [2] ; da Silva, Rafael P. [2] ; Di Mascio, Paolo [1] ; Gualano, Bruno [2] ; Artioli, Guilherme G. [2] ; Medeiros, Marisa H. G. [1]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Inst Quim, Dept Bioquim, Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Fac Med, Div Reumatol, Appl Physiol & Nutr Res Grp, Escola Educ Fis & Esp, Sao Paulo, SP - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: REDOX BIOLOGY; v. 18, p. 222-228, SEP 2018.
Citações Web of Science: 5
Resumo

Previous studies have demonstrated that exercise results in reactive aldehyde production and that beta-alanine supplementation increases carnosine content in skeletal muscle. However, little is known about the influence exercise and beta-alanine supplementation have on the formation of carnosine-aldehydes. The goal of the present study was to monitor the formation of carnosine-aldehyde adducts, following high-intensity intermittent exercise, before and after beta-alanine supplementation. Vastus lateralis biopsy samples were taken from 14 cyclists, before and after a 28 day beta-alanine supplementation, following 4 bouts of a 30 s all-out cycling test, and carnosine and CAR-aldehyde adducts {[}carnosine-acrolein, CAR-ACR (m/z 303), carnosine-4-hydroxy-2-hexenal, CAR-HHE (m/z 341) and carnosine-4-hydroxy-2-nonenal, CAR-HNE (m/z 383)] were quantified by HPLC-MS/MS. beta-alanine supplementation increased muscle carnosine content by similar to 50% (p = 0.0001 vs. Pre-Supplementation). Interestingly, there was a significant increase in post-exercise CAR-ACR content following beta-alanine supplementation (p < 0.001 vs. post-exercise before supplementation), whereas neither exercise alone nor supplementation alone increased CAR-ACR formation. These results suggest that carnosine functions as an acrolein-scavenger in skeletal muscle. Such a role would be relevant to the detoxification of this aldehyde formed during exercise, and appears to be enhanced by beta-alanine supplementation. These novel findings not only have the potential of directly benefiting athletes who engage in intensive training regimens, but will also allow researchers to explore the role of muscle carnosine in detoxifying reactive aldehydes in diseases characterized by abnormal oxidative stress. (AU)

Processo FAPESP: 14/11948-8 - Vida sem carnosina: desenvolvimento e caracterização de um modelo de rato nocaute para estudo do papel fisiológico da carnosina e suas implicações para o exercício físico e metabolismo muscular
Beneficiário:Guilherme Giannini Artioli
Linha de fomento: Auxílio à Pesquisa - Apoio a Jovens Pesquisadores
Processo FAPESP: 13/14746-4 - Metabolismo da carnosina no músculo esquelético: um estudo de múltiplas abordagens
Beneficiário:Bruno Gualano
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 13/07937-8 - Redoxoma
Beneficiário:Ohara Augusto
Linha de fomento: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
Processo FAPESP: 16/24761-9 - Esclerose Lateral Amiotrófica: estudo dos mecanismos associados a efeitos da carnosina
Beneficiário:Ana Helena Sales de Oliveira
Linha de fomento: Bolsas no Brasil - Pós-Doutorado