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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Exercise and beta-alanine supplementation on carnosine-acrolein adduct in skeletal muscle

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Author(s):
Carvalho, Victor H. [1] ; Oliveira, Ana H. S. [1] ; de Oliveira, Luana F. [2] ; da Silva, Rafael P. [2] ; Di Mascio, Paolo [1] ; Gualano, Bruno [2] ; Artioli, Guilherme G. [2] ; Medeiros, Marisa H. G. [1]
Total Authors: 8
Affiliation:
[1] Univ Sao Paulo, Inst Quim, Dept Bioquim, Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Fac Med, Div Reumatol, Appl Physiol & Nutr Res Grp, Escola Educ Fis & Esp, Sao Paulo, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: REDOX BIOLOGY; v. 18, p. 222-228, SEP 2018.
Web of Science Citations: 6
Abstract

Previous studies have demonstrated that exercise results in reactive aldehyde production and that beta-alanine supplementation increases carnosine content in skeletal muscle. However, little is known about the influence exercise and beta-alanine supplementation have on the formation of carnosine-aldehydes. The goal of the present study was to monitor the formation of carnosine-aldehyde adducts, following high-intensity intermittent exercise, before and after beta-alanine supplementation. Vastus lateralis biopsy samples were taken from 14 cyclists, before and after a 28 day beta-alanine supplementation, following 4 bouts of a 30 s all-out cycling test, and carnosine and CAR-aldehyde adducts {[}carnosine-acrolein, CAR-ACR (m/z 303), carnosine-4-hydroxy-2-hexenal, CAR-HHE (m/z 341) and carnosine-4-hydroxy-2-nonenal, CAR-HNE (m/z 383)] were quantified by HPLC-MS/MS. beta-alanine supplementation increased muscle carnosine content by similar to 50% (p = 0.0001 vs. Pre-Supplementation). Interestingly, there was a significant increase in post-exercise CAR-ACR content following beta-alanine supplementation (p < 0.001 vs. post-exercise before supplementation), whereas neither exercise alone nor supplementation alone increased CAR-ACR formation. These results suggest that carnosine functions as an acrolein-scavenger in skeletal muscle. Such a role would be relevant to the detoxification of this aldehyde formed during exercise, and appears to be enhanced by beta-alanine supplementation. These novel findings not only have the potential of directly benefiting athletes who engage in intensive training regimens, but will also allow researchers to explore the role of muscle carnosine in detoxifying reactive aldehydes in diseases characterized by abnormal oxidative stress. (AU)

FAPESP's process: 14/11948-8 - Life without carnosine: development and characterization of a KO rat model for studying the physiological role of carnosine and its implications to physical exercise and muscle metabolism
Grantee:Guilherme Giannini Artioli
Support Opportunities: Research Grants - Young Investigators Grants
FAPESP's process: 16/24761-9 - Amyotrophic Lateral Sclerosis: study of the mechanisms associated with carnosine effects
Grantee:Ana Helena Sales de Oliveira
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 13/14746-4 - Carnosine metabolism in skeletal muscle: a multi-approach study
Grantee:Bruno Gualano
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 13/07937-8 - Redoxome - Redox Processes in Biomedicine
Grantee:Ohara Augusto
Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC