Integration of genome wide association studies and whole genome sequencing provides novel insights into fat deposition in chicken

Autor(es)

Moreira, Gabriel Costa Monteiro ^[1] ; Boschiero, Clarissa ^[1] ; Mello Cesar, Aline Silva ^[1] ; Reecy, James M. ^[2] ; Godoy, Thais Fernanda ^[1] ; Pertille, Fabio ^[1] ; Ledur, Monica Correa ^[3] ; Meira Tavares Moura, Ana Silvia Alves ^[4] ; Garrick, Dorian J. ^[5] ; Coutinho, Luiz Lehmann ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Anim Sci, Piracicaba, SP - Brazil

^[2] Iowa State Univ, Dept Anim Sci, Ames, IA - USA

^[3] Embrapa Suinos & Aves, Concordia, SC - Brazil

^[4] Sao Paulo State Univ UNESP, FMVZ, Botucatu, SP - Brazil

^[5] Massey Univ, Sch Agr, Hamilton - New Zealand

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 8, NOV 1 2018.
• Citações Web of Science: 4

Resumo

Excessive fat deposition is a negative factor for poultry production because it reduces feed efficiency, increases the cost of meat production and is a health concern for consumers. We genotyped 497 birds from a Brazilian F-2 Chicken Resource Population, using a high-density SNP array (600 K), to estimate the genomic heritability of fat deposition related traits and to identify genomic regions and positional candidate genes (PCGs) associated with these traits. Selection signature regions, haplotype blocks and SNP data from a previous whole genome sequencing study in the founders of this chicken F2 population were used to refine the list of PCGs and to identify potential causative SNPs. We obtained high genomic heritabilities (0.43-0.56) and identified 22 unique QTLs for abdominal fat and carcass fat content traits. These QTLs harbored 26 PCGs involved in biological processes such as fat cell differentiation, insulin and triglyceride levels, and lipid biosynthetic process. Three of these 26 PCGs were located within haplotype blocks there were associated with fat traits, five overlapped with selection signature regions, and 12 contained predicted deleterious variants. The identified QTLs, PCGs and potentially causative SNPs provide new insights into the genetic control of fat deposition and can lead to improved accuracy of selection to reduce excessive fat deposition in chickens. (AU)

• Processo FAPESP: 15/00616-7 - Identificação e caracterização de CNVs no genoma da galinha e associação com desenvolvimento de músculo de peito
• Beneficiário: Thaís Fernanda Godoy
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 14/08704-0 - Identificação de locos de interesse zootécnico na galinha doméstica
• Beneficiário: Luiz Lehmann Coutinho
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 14/21380-9 - Estudo de associação ampla do genoma para deposição de gordura e rendimento de carcaça em galinhas
• Beneficiário: Gabriel Costa Monteiro Moreira
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 16/20440-3 - Análise epigenética de perfil de metilação em frangos submetidos à condições de estresse no ambiente de produção
• Beneficiário: Fábio Pértille
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 16/00569-1 - Imputação e associação genômica ampla utilizando haplótipos para gordura abdominal em galinhas.
• Beneficiário: Gabriel Costa Monteiro Moreira
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado