Recombination Located over 2A-2B Junction Ribosome Frameshifting Region of Saffold Cardiovirus

Autor(es):Mostrar menos -

da Costa, Antonio Charlys ^[1] ; Luchs, Adriana ^[2] ; de Padua Milagres, Flavio Augusto ^{[3, 4, 5, 6]} ; Komninakis, Shirley Vasconcelos ^{[7, 8]} ; Gill, Danielle Elise ^[1] ; Brito Sayao Lobato, Marcia Cristina Alves ^{[3, 5]} ; Brustulin, Rafael ^{[3, 4, 5]} ; das Chagas, Rogerio Togisaki ^{[3, 5]} ; Neves dos Santos Abrao, Maria de Fatima ^{[3, 5]} ; de Deus Alves Soares, Cassia Vitoria ^{[3, 5]} ; Deng, Xutao ^{[9, 10]} ; Sabino, Ester Cerdeira ^{[1, 6]} ; Delwart, Eric ^{[9, 10]} ; Leal, Elcio ^[11]

Afiliação do(s) autor(es):Mostrar menos -

^[1] Univ Sao Paulo, Inst Trop Med, BR-05403000 Sao Paulo - Brazil

^[2] Adolfo Lutz Inst, Virol Ctr, Enter Dis Lab, BR-01246000 Sao Paulo - Brazil

^[3] Secretary Hlth Tocantins, BR-77453000 Tocantins - Brazil

^[4] Fed Univ Tocantins, Inst Biol Sci, BR-77001090 Tocantins - Brazil

^[5] Publ Hlth Lab Tocantins State LACEN TO, BR-77016330 Tocantins - Brazil

^[6] Univ Sao Paulo, Fac Med, LIM-46, BR-01246903 Sao Paulo - Brazil

^[7] ABC, Fac Med, Postgrad Program Hlth Sci, BR-09060870 Santo Andre - Brazil

^[8] Univ Fed Sao Paulo, Retrovirol Lab, BR-04023062 Sao Paulo - Brazil

^[9] Univ Calif San Francisco, Dept Lab Med, San Francisco, CA 94143 - USA

^[10] Blood Syst Res Inst, San Francisco, CA 94143 - USA

^[11] Fed Univ Para, Inst Biol Sci, BR-66075000 Belem, Para - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Viruses-Basel; v. 10, n. 10 OCT 2018.
• Citações Web of Science: 2

Resumo

Here we report the nearly full-length genome of a recombinant Saffold virus strain (SAFV-BR-193) isolated from a child with acute gastroenteritis. Evolutionary analysis performed using all available near-full length Saffold picornavirus genomes showed that the breakpoint found in the Brazilian strain (SAFV-BR-193) is indeed a recombination hotspot. Notably, this hotspot is located just one nucleotide after the ribosomal frameshift GGUUUUU motif in the SAFV genome. Empirical studies will be necessary to determine if this motif also affects the binding affinity of RNA-dependent RNA-polymerase (RdRp) and therefore increases the changes of RdRp swap between molecules during the synthesis of viral genomes. (AU)

• Processo FAPESP: 15/12944-9 - Investigando a evolução de cepas animais de rotavírus infectando humanos
• Beneficiário: Adriana Luchs
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/01735-2 - Metagenômica viral de Dengue, Chikungunya e Zika vírus: acompanhar, explicar e prever a transmissão e distribuição espaço-temporal no Brasil
• Beneficiário: Ester Cerdeira Sabino
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/00021-9 - Metagenômica viral da Dengue, Chikungunya e Zika: acompanhar, explicar e prever a transmissão e distribuição espaço-temporal no Brasil
• Beneficiário: Antonio Charlys da Costa
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado