Recombination Located over 2A-2B Junction Ribosome Frameshifting Region of Saffold Cardiovirus

Author(s):Show less -

da Costa, Antonio Charlys ^[1] ; Luchs, Adriana ^[2] ; de Padua Milagres, Flavio Augusto ^{[3, 4, 5, 6]} ; Komninakis, Shirley Vasconcelos ^{[7, 8]} ; Gill, Danielle Elise ^[1] ; Brito Sayao Lobato, Marcia Cristina Alves ^{[3, 5]} ; Brustulin, Rafael ^{[3, 4, 5]} ; das Chagas, Rogerio Togisaki ^{[3, 5]} ; Neves dos Santos Abrao, Maria de Fatima ^{[3, 5]} ; de Deus Alves Soares, Cassia Vitoria ^{[3, 5]} ; Deng, Xutao ^{[9, 10]} ; Sabino, Ester Cerdeira ^{[1, 6]} ; Delwart, Eric ^{[9, 10]} ; Leal, Elcio ^[11]

Affiliation:Show less -

^[1] Univ Sao Paulo, Inst Trop Med, BR-05403000 Sao Paulo - Brazil

^[2] Adolfo Lutz Inst, Virol Ctr, Enter Dis Lab, BR-01246000 Sao Paulo - Brazil

^[3] Secretary Hlth Tocantins, BR-77453000 Tocantins - Brazil

^[4] Fed Univ Tocantins, Inst Biol Sci, BR-77001090 Tocantins - Brazil

^[5] Publ Hlth Lab Tocantins State LACEN TO, BR-77016330 Tocantins - Brazil

^[6] Univ Sao Paulo, Fac Med, LIM-46, BR-01246903 Sao Paulo - Brazil

^[7] ABC, Fac Med, Postgrad Program Hlth Sci, BR-09060870 Santo Andre - Brazil

^[8] Univ Fed Sao Paulo, Retrovirol Lab, BR-04023062 Sao Paulo - Brazil

^[9] Univ Calif San Francisco, Dept Lab Med, San Francisco, CA 94143 - USA

^[10] Blood Syst Res Inst, San Francisco, CA 94143 - USA

^[11] Fed Univ Para, Inst Biol Sci, BR-66075000 Belem, Para - Brazil

• Document type: Journal article
• Source: Viruses-Basel; v. 10, n. 10 OCT 2018.
• Web of Science Citations: 2

Abstract

Here we report the nearly full-length genome of a recombinant Saffold virus strain (SAFV-BR-193) isolated from a child with acute gastroenteritis. Evolutionary analysis performed using all available near-full length Saffold picornavirus genomes showed that the breakpoint found in the Brazilian strain (SAFV-BR-193) is indeed a recombination hotspot. Notably, this hotspot is located just one nucleotide after the ribosomal frameshift GGUUUUU motif in the SAFV genome. Empirical studies will be necessary to determine if this motif also affects the binding affinity of RNA-dependent RNA-polymerase (RdRp) and therefore increases the changes of RdRp swap between molecules during the synthesis of viral genomes. (AU)

• FAPESP's process: 15/12944-9 - Investigating the evoltution of animal rotavirus strains infecting humans
• Grantee: Adriana Luchs
• Support Opportunities: Regular Research Grants
• FAPESP's process: 16/01735-2 - Viral metagenomics to track, explain and predict the transmission and spatiotemporal spread of Dengue and Chikungunya viruses in Brazil
• Grantee: Ester Cerdeira Sabino
• Support Opportunities: Regular Research Grants
• FAPESP's process: 17/00021-9 - Viral metagenomics to track, explain and predict the transmission and spatiotemporal spread of Dengue and Chikungunya viruses in Brazil
• Grantee: Antonio Charlys da Costa
• Support Opportunities: Scholarships in Brazil - Post-Doctoral