Molecular characterization of Wnt pathway and function of -catenin overexpression in medulloblastoma cell lines

Autor(es)

Geron, Lenisa ^[1] ; Salomao, Karina Bezerra ^{[2, 1]} ; Borges, Kleiton Silva ; Andrade, Augusto Faria ^[1] ; Pereira Correa, Carolina Alves ^[1] ; Scrideli, Carlos Alberto ^[2] ; Tone, Luiz Gonzaga ^{[2, 1]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Genet, Ribeirao Preto Med Sch, 3900 Bandeirantes Ave, BR-14049900 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Dept Pediat, Ribeirao Preto Med Sch, 3900 Bandeirantes Ave, BR-14049900 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Cytotechnology; v. 70, n. 6, p. 1713-1722, DEC 2018.
• Citações Web of Science: 0

Resumo

Medulloblastoma (MB) is the most common malignant childhood brain tumor. MB is currently classified into four molecular subgroups (Wnt, Shh, Group 3, and Group 4). The wingless (Wnt) pathway is responsible for embryonic development and is deregulated in MB. We analyzed the activation of the Wnt pathway in MB cell lines and its correlation with the Shh pathway, with emphasis on the importance of cellular characterization. Transient -catenin transfection led to an increase in the -catenin gene and protein expression in MB cell lines. Wnt pathway activation resulted in a reduced number of colonies in all cell lines studied and a significant increase in the G2/M cell cycle phase only in ONS-76 cells. Regarding the Shh pathway, transfection caused a reduced expression of the PTCH1 and SMO genes only in the UW473 cells. Further studies are needed to understand the mechanism underlying the molecular events associated with the effects of Wnt activation in MB. (AU)

• Processo FAPESP: 14/07124-0 - Estudo da modulação da via Wnt pelo inibidor de Aurora-quinases AMG 900 em linhagens celulares de meduloblastoma pediátrico
• Beneficiário: Lenisa Geron
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 14/20341-0 - Interação entre alvos terapêuticos emergentes e vias de desenvolvimento associadas à tumorigênese: ênfase em neoplasias da criança e do adolescente
• Beneficiário: Luiz Gonzaga Tone
• Modalidade de apoio: Auxílio à Pesquisa - Temático