In vivo biocompatibility of an interim denture resilient liner containing antifungal drugs

Autor(es)

Hotta, Juliana ^[1] ; Garlet, Gustavo Pompermeier ^[2] ; Cestari, Tania Mary ^[2] ; Mello Lima, Jozely Francisca ^[3] ; Porto, Vinicius Carvalho ^[1] ; Urban, Vanessa Migliorini ^[4] ; Neppelenbroek, Karin Hermana ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Bauru Sch Dent, Dept Prosthodont & Periodont, Bauru, SP - Brazil

^[2] Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, Bauru - Brazil

^[3] Paulo Picanco Fac, Sch Dent, Dept Dent, Fortaleza, Ceara - Brazil

^[4] Univ Estadual Ponta Grossa, Dept Dent, Ponta Grossa - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF PROSTHETIC DENTISTRY; v. 121, n. 1, p. 135-142, JAN 2019.
• Citações Web of Science: 1

Resumo

Statement of problem. Antifungal agents incorporated into interim denture resilient liners have been suggested as an adjunct treatment for denture stomatitis (DS). However, before applying this protocol to humans, biocompatibility analysis of such drugs in animal models is required. Purpose. The purpose of this animal study was to evaluate the in vivo biocompatibility of an interim resilient liner modified with minimum inhibitory concentrations (MICs) of antifungal drugs for Candida albicans biofilm. Materials and methods. Sixty Wistar rats were divided into 6 groups (n=5): PC=positive control/no protocol; IOD (intraoral device)=rats using an acrylic resin palatal device (PD); Tru=rats using a PD relined with Trusoft; and Ny (nystatin), Chx (chlorhexidine diacetate), and Ke (ketoconazole) groups=rats using a PD relined with Trusoft + drug MICs. The rats were sacrificed at 7 or 14 days of trial. Histopathological qualitative analysis was performed by comparing photomicrographs of histological sections of the intermolar region. Morphological changes in the epithelium and keratin were quantitatively analyzed by computerized planimetry, and data were analyzed by using 2-way ANOVA and the Tukey HSD test (alpha=.05). Results. Quantitative analysis showed that only PD containing Ke significantly decreased the thickness and area of the keratin compared with the other groups (P<.001), which showed no differences between each other (P>.05). These results agreed with those of qualitative analysis. Conclusion. Incorporation of MICs of Ny and Chx in Trusoft did not induce histopathological changes in the rat palatal mucosa, suggesting the in vivo biocompatibility of this DS treatment. (AU)

• Processo FAPESP: 13/10400-6 - Modificação de reembasadores resilientes temporários para base de próteses com agentes antimicrobianos: estudos in vitro e in vivo
• Beneficiário: Jozely Francisca Mello Lima
• Modalidade de apoio: Bolsas no Brasil - Programa Capacitação - Treinamento Técnico
• Processo FAPESP: 12/24291-1 - Modificação de reembasadores resilientes temporários para base de próteses com agentes antimicrobianos: estudos in vitro e in vivo
• Beneficiário: Vinicius Rizzo Marques
• Modalidade de apoio: Bolsas no Brasil - Programa Capacitação - Treinamento Técnico
• Processo FAPESP: 12/11074-2 - Modificação de reembasadores resilientes temporários para base de próteses com agentes antimicrobianos: estudos in vitro e in vivo
• Beneficiário: Karin Hermana Neppelenbroek
• Modalidade de apoio: Auxílio à Pesquisa - Regular